The Effect of Pretreatment of Aerobic Training on Some of the Pulmonary Stress Indices against the Toxicity of Doxorubicin

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Sport Physiology, Payame Noor University, Tehran, Iran

2 Professor, Department of Sport Physiology, School of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran

Abstract

Background: The purpose of this study was to determine the effect of pretreatment of aerobic training on stress and inflammatory indices [heat shock protein-70 (HSP70), superoxide dismutase (SOD), malondialdehyde (MDA) and C-reactive protein (CRP)] induced by doxorubicin in lung tissue of aging male rats.Methods: 16 male rats were randomly divided into aging + doxorubicin (AD) and aging + doxorubicin + training (ADT) groups. The training group ran on a treadmill for three weeks at speeds of 15-17 m/minute for 25-39 minutes and five sessions a week. Subjects received doxorubicin (20 mg/kg) 24 hours after the last training session. The biopsy was taken 48 hours later. Data were analyzed using one-way ANOVA and Scheffe post hoc test via SPSS software.Findings: Three weeks of the aerobic training only resulted in a significant increase of HSP70 and SOD, and decrease of MDA in the lung tissue in the training group compared to the control group.Conclusion: Doing exercises before treatment with doxorubicin appear to be effective in elderly people, probably due to changes in the protective proteins of the cell in inhibiting or reducing oxidative damage.

Keywords

References

  1. Wynder EL, Hoffmann D. Smoking and lung cancer: Scientific challenges and opportunities. Cancer Res 1994; 54(20): 5284-95.
  2. Bello B, Fadahun O, Kielkowski D, Nelson G. Trends in lung cancer mortality in South Africa: 1995-2006. BMC Public Health 2011; 11: 209.
  3. Carvalho FS, Burgeiro A, Garcia R, Moreno AJ, Carvalho RA, Oliveira PJ. Doxorubicin-induced cardiotoxicity: From bioenergetic failure and cell death to cardiomyopathy. Med Res Rev 2014; 34(1): 106-35.
  4. Kelleni MT, Amin EF, Abdelrahman AM. Effect of metformin and sitagliptin on doxorubicin-induced cardiotoxicity in rats: Impact of oxidative stress, inflammation, and apoptosis. J Toxicol 2015; 2015: 424813.
  5. Marques-Aleixo I, Santos-Alves E, Mariani D, Rizo-Roca D, Padrao AI, Rocha-Rodrigues S, et al. Physical exercise prior and during treatment reduces sub-chronic doxorubicin-induced mitochondrial toxicity and oxidative stress. Mitochondrion 2015; 20: 22-33.
  6. Kirkham AA, Davis MK. Exercise prevention of cardiovascular disease in breast cancer survivors. J Oncol 2015; 2015: 917606.
  7. Machado NG, Baldeiras I, Pereira GC, Pereira SP, Oliveira PJ. Sub-chronic administration of doxorubicin to Wistar rats results in oxidative stress and unaltered apoptotic signaling in the lung. Chem Biol Interact 2010; 188(3): 478-86.
  8. Ertekin MV, Kocer I, Karslioglu I, Taysi S, Gepdiremen A, Sezen O, et al. Effects of oral Ginkgo biloba supplementation on cataract formation and oxidative stress occurring in lenses of rats exposed to total cranium radiotherapy. Jpn J Ophthalmol 2004; 48(5): 499-502.
  9. Rezaeyan A, Haddadi G H, Hosseinzadeh M. Evaluating superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (mda) and the histological changes of the lung tissue after γ-Irradiation in Rats. J Fasa Univ Med Sci 2016; 6(2): 235-45. [In Persian].
  10. Sadat-Hoseini SK, Dabidi Roshan V. The interactive effects of two forced and voluntary exercise training method and Nanocurcumin supplement on doxorubicin-induced hepatotoxicity in aging induced by D-galactose. Tehran Univ Med J 2017; 74(11): 807-16. [In Persian].
  11. Noaman E, Zahran AM, Kamal AM, Omran MF. Vitamin E and selenium administration as a modulator of antioxidant defense system: biochemical assessment and modification. Biol Trace Elem Res 2002; 86(1): 55-64.
  12. Molvarec A, Prohaszka Z, Nagy B, Kalabay L, Szalay J, Fust G, et al. Association of increased serum heat shock protein 70 and C-reactive protein concentrations and decreased serum alpha(2)-HS glycoprotein concentration with the syndrome of hemolysis, elevated liver enzymes, and low platelet count. J Reprod Immunol 2007; 73(2): 172-9.
  13. Ascensao A, Magalhaes J, Soares J, Ferreira R, Neuparth M, Marques F, et al. Endurance training attenuates doxorubicin-induced cardiac oxidative damage in mice. Int J Cardiol 2005; 100(3): 451-60.
  14. Lien CY, Jensen BT, Hydock DS, Hayward R. Short-term exercise training attenuates acute doxorubicin cardiotoxicity. J Physiol Biochem 2015; 71(4): 669-78.
  15. Kopalli SR, Won YJ, Hwang SY, Cha KM, Kim SY, Han CK, et al. Korean red ginseng protects against doxorubicin-induced testicular damage: An experimental study in rats. J Funct Foods 2016; 20: 96-107.
  16. Ashrafi J, Dabidi Roshan V, Mahjoub P. Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat. Afr J Pharm Pharmacol 2012; 6(31): 2380-8.
  17. Dolci A, Dominici R, Cardinale D, Sandri MT, Panteghini M. Biochemical markers for prediction of chemotherapy-induced cardiotoxicity: Systematic review of the literature and recommendations for use. Am J Clin Pathol 2008; 130(5): 688-95.
  18. Letchford K, Burt H. A review of the formation and classification of amphiphilic block copolymer nanoparticulate structures: Micelles, nanospheres, nanocapsules and polymersomes. Eur J Pharm Biopharm 2007; 65(3): 259-69.
  19. Mohamad RH, El-Bastawesy AM, Zekry ZK, Al-Mehdar HA, Al-Said MG, Aly SS, et al. The role of Curcuma longa against doxorubicin (adriamycin)-induced toxicity in rats. J Med Food 2009; 12(2): 394-402.
  20. Kumar V, Abbas A, Aster J. Dependable, current, and complete, Robbins and Cotran Pathologic Basis of Disease. 8th ed. Philadelphia, PA: Saunders/Elsevier; 2010. p. 60.
  21. Blech DM, Borders CL. Hydroperoxide anion, HO-2, is an affinity reagent for the inactivation of yeast Cu,Zn superoxide dismutase: Modification of one histidine per subunit. Arch Biochem Biophys 1983; 224(2): 579-86.
  22. Zolfagharzadeh F, Roshan VD. Pretreatment hepatoprotective effect of regular aerobic training against hepatic toxicity induced by Doxorubicin in rats. Asian Pac J Cancer Prev 2013; 14(5): 2931-6.
  23. Zolfagharzadeh F, Dabidi Roshan V, Hajizadeh M. Pretreatment Effect of Threeand Sixweeks Aerobic Exercise on Acute Doxorubicin-Induced Hepatic Stress. Olympic Modern 2015; 1(2): 117-28.
  24. El-Sayed M, El-Azeem A, Afify A, Shabana M, Ahmed H. Cardioprotective effects of Curcuma longa L. extracts against doxorubicin-induced cardiotoxicity in rats. J Med Plants Res 2010; 5(17): 4049-58.
  25. Gibson OR, Mee JA, Taylor L, Tuttle JA, Watt PW, Maxwell NS. Isothermic and fixed-intensity heat acclimation methods elicit equal increases in Hsp72 mRNA. Scand J Med Sci Sports 2015; 25 Suppl 1: 259-68.
  26. Powers SK, Duarte J, Kavazis AN, Talbert EE. Reactive oxygen species are signalling molecules for skeletal muscle adaptation. Exp Physiol 2010; 95(1): 1-9.
  27. 27. Dabidi-Roshan V, Rahnama N, Abdi Hamzehkolaei H, Fallah Mohammadi Z. Heat shock protein responses to eccentric weight or treadmill exercise in active young females. Sport Sci Health 2009; 5: 75-80.
  28. ShirinbayanV., Dabidi RoshanV., MahjoubS. The therapeutic effect of endurance training on adriamycin-induced cardiac stress in rats. Iranian Journal of Health and Physical Activity 2013; 4(2): 8-17.
  29. Lee Y, Kwon I, Jang Y, Cosio-Lima L, Barrington P. Endurance exercise attenuates doxorubicin-induced cardiotoxicity. Med Sci Sports Exerc 2020; 52(1): 25-36.
  30. Lee M, Cho HS, Yoon KJ, Lee W, Moon HY. Exercise-induced changes of gene expression in the cerebellum of aged mice. Biochem Biophys Res Commun 2020; 521(4): 952-6.
Journal of Isfahan Medical School
Volume 37, Issue 559 - Serial Number 559
November and December 2020
Pages 1422-1427

Files

History

  • Receive Date: 01 June 2019
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics