Evaluation of miR-143 and miR-145 Expression in Tumors and Tumor Margins in Patients with Colorectal Cancer

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Biology, Urmia Branch, Islamic Azad University, Urmia, Iran

2 Associate Professor, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

3 Assistant Professor, Department of Biology, Urmia Branch, Islamic Azad University, Urmia, Iran

Abstract

Background: microRNAs are non-coding RNAs with about 22 nucleotides in length which modulate gene expression by binding to the gene target mRNA; they have eminent role as oncomiRs or microRNAs tumor suppressors. Changes in microRNAs expression have a radical role in pathobiology of different cancers. In this research, miR-143 and miR-145 expression in the tumor cells and tumor margins of patients with colorectal cancer were evaluated.Methods: Tumor cells and tumor margins were examined after being biopsied from 30 patients with colorectal cancer. Total RNA content was extracted from both tissue types with TRIzol reagent and then, complementary DNA (cDNA) of miRNAs were synthesized. microRNAs expression level was assessed through reverse transcription-polymerase chain reaction (RT-PCR) method using the SYBR Green. For statistical analysis, GraphPad Prism software was applied to compare the expression level of the microRNAs between tumor cells and tumor margins.Findings: The expression level of miR-143 and miR-145 were significantly lower in tumors compared to the margins (P < 0.0001 for both).Conclusion: Expression level of miR-143 and miR-145 were down-regulated in tumors compared to the margins. Lower expression level revealed that miR-143 and miR-145 can be utilized as diagnostic biomarkers in colorectal cancer. This may also introduce these microRNAs as colorectal cancer therapeutic targets.

Keywords

References

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Journal of Isfahan Medical School
Volume 35, Issue 452 - Serial Number 452
September and October 2017
Pages 1504-1508

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History

  • Receive Date: 07 March 2017
  • Revise Date: 02 November 2024
  • Accept Date: 06 April 2022

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