Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 PhD Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

3 Assistant Professor, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

4 Associate Professor, Department of Biochemistry, School of Medicine AND Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

Background: Sialic acid (SA) is presented in all cells membrane of vertebrates, and its level in the human brain is much higher than other body tissues. Studies have shown that, in addition to oxidative stress, increasing the amount of SA can also lead to the development of neurological diseases including Alzheimer's disease. Therefore, the present study was designed to evaluate the effect of SA on malondialdehyde (MDA) production levels, as a lipid peroxidation product, in human astroglia.Methods: The human astroglias were cultured in Dulbecco's Modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), and cells were treated with different doses of SA. MDA was measured using thiobarbituric acid (TBA) protocol, and the results were analyzed using SPSS software.Findings: The production of MDA in treated cells with 200, 500, and 1000 μg/ml of SA significantly increased compared to the control group. It also significantly increased when the cells were treated with 200 μg/ml of SA at 12, 16, and 24 hours incubation.Conclusion: Many studies have been conducted on neurological disorders; however their mechanism of occurrence has not yet been fully elucidated. With regard to the role of SA in inflammation, our results suggest that SA can cause pathological conditions and oxidative stress followed by MDA elevation; which is effective in the development of neurological disorders like Alzheimer's. The role of SA and its effects need further studies.

Keywords

References

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Journal of Isfahan Medical School
Volume 37, Issue 520 - Serial Number 520
January and February 2019
Pages 263-269

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History

  • Receive Date: 11 April 2019
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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