Association of rs6065668 Polymorphism and TOX2 Gene Expression with Colorectal Cancer Risk

Document Type : Original Article(s)

Authors

1 MSc, Department of Science, School of Basic Sciences, Dezful Branch, Islamic Azad University, Dezful, Iran

2 Assistant Professor, Department of Nursing and Midwifery, Dezfoul Branch, Islamic Azad University, Dezfoul, Iran

3 Assistant Professor, Department of Genetics and Molecular Biology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran

Abstract

Background: Colorectal cancer (CRC) is the third most common cancer and the fourth cause of death due to cancer. Several genes with different penitence, as well as different genetic variants, have been identified in CRC risk. Based on the studies, the TOX2 gene is proposed as a candidate gene for CRC. In the present study, the relationship between rs6065668 polymorphism and TOX2 gene expression was investigated with the risk of CRC.
Methods: In the present study, 2 CC of venous blood was collected from 50 patients referred to the Imam Hassan Mojtabi Cancer Treatment Center in Dezful, and 50 healthy individuals as a control group (matched in terms of age and gender). In this study, rs6065668 polymorphism of the TOX2 gene was investigated by the High-Resolution Melting (HRM) technique and gene expression by the Real-time PCR method.
Findings: Findings: The frequency of genotypes in patients was 42% for the CC genotype, 46% for CT, and 12% for TT, and in the control group, CC, CT, and TT genotypes were 26%, 54%, and 20%, respectively, which had no significant difference between the groups and as well as the results of gene expression were not significantly different between groups and different genotypes.
Conclusion: Considering that the frequency of CC, CT, and TT genotypes as well as TOX2 gene expression did not show a statistically significant difference between CRC patients compared to healthy subjects, the results of this study showed that changes in TOX2 gene are not related to the risk of CRC.

Keywords

Main Subjects

References

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Journal of Isfahan Medical School
Volume 41, Issue 725
1st Week, September
September and October 2023
Pages 517-523

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History

  • Receive Date: 05 July 2023
  • Accept Date: 29 July 2023

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