The Therapeutic Effect of the Combination of an Electron Beam of 6 Megaelectron Volts and Cisplatin on the B16F10 Mouse Melanoma Cell Line

Document Type : Original Article (s)

Authors

1 MSc Student in Medical Physics, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences Introduction, Isfahan, Iran

3 Professor, Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Assistant Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 Assistant Professor, Department of Medical Nanotechnology, Islamic Azad University, Tehran, Iran

Abstract

Background: Melanoma cancer is the most dangerous and deadly skin cancer, for which preventive treatments such as surgery, radiation therapy, and chemotherapy have been suggested. In this study, we tried to investigate the effect of the simultaneous use of electron beam and cisplatin drug.
Methods: Cells in DMEM-F12 culture medium containing 10% fetal bovine serum (FBS) and streptomycin and penicillin antibiotics in an incubator at 37°C and 5% carbon dioxide in the amount of 2×104 They were cultured to perform the MTT test. The cytotoxic effect of Cisplatin with concentrations of 0.6, 1.5, 5, 10, and 20 μg/ml and electron beam of 1, 2, and 3 Gy was measured separately by MTT test and, then the effect of the combined treatment on cells was measured using all drug concentrations and radiation doses. The statistical tests for drugs, radiation, and their combination were fully performed.
Findings: Based on statistical tests, increasing the concentration of the drug alone or in combination with an electron beam increased the level of toxicity, and this significant increase was reported. Also, increasing the dose of the drug separately and in combination with the drug significantly increased the toxicity gave.
Conclusion: The combination of two treatment methods can prevent the use of high concentrations of drugs or high doses of electron beams and significantly reduce the application limitations and side effects of drugs and radiation.

Highlights

Javad Garshad: PubMed, Google Scholar

Ahmad Shanei: PubMed, Google Scholar

Iraj Abedi: PubMed, Google Scholar

Keywords

Main Subjects

References

  1. Slominski RM, Zmijewski MA, Slominski AT. The role of melanin pigment in melanoma. Exp Dermatol 2015; 24(4): 258-9.
  2. Arnold M, Holterhues C, Hollestein LM, Coebergh JWW, Nijsten T, Pukkala E, et al. Trends in incidence and predictions of cutaneous melanoma across Europe up to 2015. J Eur Acad Dermatology Venereol 2014; 28(9): 1170-8.
  3. Arnold M, Singh D, Laversanne M, Vignat J, Vaccarella S, Meheus F, et al. Global Burden of Cutaneous Melanoma in 2020 and Projections to 2040. JAMA Dermatology 2022; 158(5): 495-503.
  4. Adamson AS, Zhou L, Baggett CD, Thomas NE, Meyer A-M. Association of delays in surgery for melanoma with insurance type. JAMA dermatology 2017; 153(11): 1106-13.
  5. Yuan C, Zhang D, Tang Y, Guo Z, Lin K, Yu Y, et al. Fibrous dressing containing bioactive glass with combined chemotherapy and wound healing promotion for post-surgical treatment of melanoma. Biomater Adv 2023; 149: 213387.
  6. Tagliaferri L, Lancellotta V, Fionda B, Mangoni M, Casà C, Di Stefani A, et al. Immunotherapy and radiotherapy in melanoma: a multidisciplinary comprehensive review. Hum Vaccin Immunother 2022; 18(3): 1903827.
  7. Gorgizadeh M, Azarpira N, Lotfi M, Daneshvar F, Salehi F, Sattarahmady N. Sonodynamic cancer therapy by a nickel ferrite/carbon nanocomposite on melanoma tumor: In vitro and in vivo studies. Photodiagnosis Photodyn Ther 2019; 27: 27-33.
  8. Wyld L, Audisio RA, Poston GJ. The evolution of cancer surgery and future perspectives. Nat Rev Clin Oncol 2015; 12(2): 115-24.
  9. Reisz JA, Bansal N, Qian J, Zhao W, Furdui CM. Effects of ionizing radiation on biological molecules—mechanisms of damage and emerging methods of detection. Antioxid Redox Signal 2014; 21(2): 260-92.
  10. Strasburg S, Hinshelwood DD, Schumer JW, Mosher D, Ottinger PF, Fernsler RF, et al. Intense electron-beam ionization physics in air. Phys Plasmas 2003; 10(9): 3758-69.
  11. Fan X, Niemira BA. Gamma ray, electron beam, and X-ray irradiation. Food Saf Eng 2020; 471-92.
  12. Wróblewska-Łuczka P, Grabarska A, Florek-Łuszczki M, Plewa Z, Łuszczki JJ. Synergy, additivity, and antagonism between cisplatin and selected coumarins in human melanoma cells. Int J Mol Sci 2021; 22(2): 537.
  13. Achkar IW, Abdulrahman N, Al-Sulaiti H, Joseph JM, Uddin S, Mraiche F. Cisplatin based therapy: the role of the mitogen activated protein kinase signaling pathway. J Transl Med. 2018; 16(1): 96.
  14. Cui C, Yang J, Li X, Liu D, Fu L, Wang X. Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance. Mol Cancer 2020; 19: 1-16.
  15. Liao C-K, Kuo Y-T, Hsu Y-J, Chern Y-J, Yu Y-L, Lin Y-C, et al. Effect of short-course radiotherapy followed by oxaliplatin-based consolidation chemotherapy on organ preservation in locally advanced rectal cancer. Int J Colorectal Dis 2023; 38(1): 92.
  16. Han J, Zakeri K, Raab G, Hesse J, Shamseddine A, Chen L, et al. Concurrent carboplatin and paclitaxel definitive radiation therapy for locally advanced head and neck cancer. Head Neck 2023; 45(9): 2207-16.
  17. Saba NF, Sukari A, Forget F, Popovtzer A, Perez JR, Park JH, et al. HyperlynX: A phase 1b safety study of xevinapant, weekly cisplatin, and radiotherapy in patients with unresected locally advanced squamous cell carcinoma of the head and neck. Int J Radiat Oncol Biol Phys 2024; 118(5): e21.
  18. Serša G, Štabuc B, Cemazar M, Miklavcic D, Rudolf Z. Electrochemotherapy with cisplatin: clinical experience in malignant melanoma patients. Clin Cancer Res 2000; 6(3): 863-7.
  19. Reisz JA, Bansal N, Qian J, Zhao W, Furdui CM. Effects of ionizing radiation on biological molecules—mechanisms of damage and emerging methods of detection. Antioxid Redox Signal 2014; 21(2): 260-92.
  20. Mortezaee K, Najafi M. Immune system in cancer radiotherapy: Resistance mechanisms and therapy perspectives. Crit Rev Oncol Hematol 2021; 157: 103180.
  21. Bukowski K, Kciuk M, Kontek R. Mechanisms of multidrug resistance in cancer chemotherapy. Int J Mol Sci. 2020;21(9):3233
  22. Liu Y, Zheng C, Huang Y, He M, Xu WW, Li B. Molecular mechanisms of chemo‐and radiotherapy resistance and the potential implications for cancer treatment. MedComm 2021; 2(3): 315-40.
  23. Nadi S, Monfared AS, Zabihi E, Mahmoudzadeh A, Eyvazzadeh N, Tahamtan R. Combined effect of iodine contrast media, cisplatin and external beam radiotherapy on anaplastic thyroid cancer cells. J Biomed Phys Eng 2019; 9(2): 217-26.

Hussain Z, Rahim MA, Jan N, Shah H, Rawas-Qalaji M, Khan S, et al. Cell membrane cloaked nanomedicines for bio-imaging and immunotherapy of cancer: Improved pharmacokinetics, cell internalization and anticancer efficacy. J Control Release 2021; 335: 130-57.

Journal of Isfahan Medical School
Volume 42, Issue 768
4th Week, July
July and August 2024
Pages 411-417

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History

  • Receive Date: 29 May 2024
  • Accept Date: 05 August 2024

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