The Effects of Coculture of Stem Cells from Human Exfoliated Deciduous Teeth (SHED) on K562 Tumor Cells

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Anatomical Sciences, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan. Iran

2 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan. Iran

3 Assistant Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan. Iran

4 PhD student, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan. Iran

5 - Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan. Iran

Abstract

Background: Due to increased prevalence of cancer in human population, the effectiveness of chemotherapy and drug (therapy) is reducing because of recurrence and drug-resistance. Therefore, scientists are trying other options such as cell-therapy. Among the cells, scientists have more focused on evaluating effect of stem cells on tumor cells. Since there were controversial reports on this effect in literature, the aim of this study was to assess the proliferation and survival of K562 tumor cells cocultured with stem cells from human exfoliated deciduous teeth (SHED).Methods: Stem cells were isolated from human exfoliated deciduous teeth and cultured K562 cells were obtained from the Pasteur institute of Iran. Stem cells and K562 were directly cocultured with each other and the ratios of K562 to stem cells were 1/10, 10/1, and 1/1. In this study, the viability and proliferation of the cells via MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and trypan blue staining.Findings: The trypan blue and MTT assay results of 48 hours coculture with ratio of 1/10 in comparison with control group did not show any significance difference. But with this ratio, at 96 hour, proliferation of K562 compared to control had reduced significantly; and in other ratios (1/1 and 1/10) at 48 and 96 hours, proliferation of K562 increased significantly (P < 0.05).Conclusion: Results of our study revealed that in this coculture, increased number of stem cells had an inhibitory effect on proliferation of K562 cells. Moreover, low numbers of stem cells not only were not able to inhibit tumor cells, but also contributed to improve their proliferation.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 304 - Serial Number 304
September and October 2014
Pages 1633-1645

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History

  • Receive Date: 09 March 2014
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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