The Effect of Pomegranate Extract on Producing Type II Collagen in Differentiation of Adipose-Derived Stem Cells into Chondrocytes

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Professor, Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences AND Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Assistant Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Cartilage tissue is avascular and has no repairing capacity. For cartilage tissue engineering, stem cells and growth factors are used. In this study, the effect of pomegranate as inducer for chondrogenesis of adipose-derived stem cells (ADSCs) was evaluated.Methods: Human adipose-derived stem cells in third passage seeded in fibrin were cultured in chondrogenic medium with pomegranate for 2 weeks. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) and western blot technique were applied for evaluation of differentiated cells.Findings: Adipose-derived stem cells differentiated into chondrocytes; and type II collagen production by differentiated cells was proved.Conclusion: Pomegranate extract is an appropriate inducer for production of type II collagen in adipose-derived stem cells.

Keywords

References

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Journal of Isfahan Medical School
Volume 35, Issue 453 - Serial Number 453
September and October 2018
Pages 1540-1545

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History

  • Receive Date: 17 September 2017
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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