Simultaneous Diagnosis of HIV-1 and HCV Infections by Nucleic Acid Sequence-Based Amplification

Document Type : Original Article (s)

Authors

1 PhD Student of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute, Tehran, Iran

2 PhD Student of Virology, Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran

3 PhD Student of Molecular Medicine, Department of Genetics, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran

Abstract

Background: Due to some problems of serological assays in diagnosis of patients infected with HIV-1 and HCV, the use of molecular assay has become widely accepted. However, molecular assays have a major drawback of high cost and dependency to expensive thermocycler instruments.Methods: This research described the development of a multiplex nucleic acid sequence-based amplification (NASBA) assay for the simultaneous or single detection of HIV-1 and HCV genomes in plasma samples. The sensitivity and specificity of this assay has been evaluated using several clinical samples.Findings: The results showed that the primers used in this assay did not have any interactions with each other or with other possible interfering agents. The analytical sensitivity of this assay for both HIV-1 and HCV was calculated as 1000 copies/ml. The clinical sensitivity and specificity of the assay were considered as 90% and 100%, respectively. Conclusion: The developed multiplex NASBA assay can be used for simultaneous or single detection of HIV-1 and HCV viruses with a suitable sensitivity and specificity. Due to easy application, multiplexity, and no need to thermocycler instruments, this assay can be used as a low cost method in laboratories with limited instruments.

Keywords

References

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Journal of Isfahan Medical School
Volume 29, Issue 172 - Serial Number 172
November and December 2012
Pages 2865-2874

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History

  • Receive Date: 16 August 2011
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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