Regulatory T-cell Profile in Early and Late Lesions of Cutaneous Leishmaniasis Due to Leishmania Major

Document Type : Original Article (s)

Authors

1 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Center for Research in Skin Disease and Leprosy, Tehran University of Medical Sciences, Tehran, Iran

5 Department of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

6 Cell Science Research Center, Royan Institute for Animal Biotechnology, The Academic Center for Education, Culture and Research (ACECR), Isfahan, Iran

7 Associate Professor, Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

8 Department of Biology, School of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shrekord, Iran

9 Associate Professor, Department of Parasitology and Mycology, Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Cutaneous leishmaniasis (CL) is a public health problem in several endemic countries. Recent studies on mouse model and a few clinical experiments showed that the type of immune response generated at the site of infection and especially balance between regulatory and effector T-cells determines the outcome of the disease toward self-limiting or long-lasting lesions. The aim of this study was to evaluate the role of natural regulatory T-cells (nTregs) in early and late cutaneous lesions of human Leishmania major (L. major) infection.Methods: Skin biopsies were collected from parasitologically proven lesions of 28 patients with cutaneous leishmaniasis, divided into two groups of early and late lesions. The causative agents were identified to be L. major. Quantitative real-time reverse transcription polymerase chain reaction (PCR) and immunofluorescent staining of biopsies were used to assess the Foxp3 mRNA expression and frequency of nTregs in two groups. Mann-Whitney U test was used to determine the significance of deferences between the two groups.Findings: Mean relative expressions of Foxp3 mRNA were 0.53 ± 0.23 and 1.26 ± 0.99 in early and late lesions, respectively, which was significantly upper in chronic lesions (P = 0.007). Parallel results were obtained in tissue staining method.Conclusion: Increased in gene expression and protein staining of nTreg markers in chronic biopsy samples indicates a role for these cells in chronic L. major induced leishmaniasis and supports the effectiveness of regulatory T cell-based immunotherapy for treatment of chronic cutaneous leishmaniasis.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 269 - Serial Number 269
November and December 2014
Pages 2326-2335

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History

  • Receive Date: 21 January 2014
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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