The Effect of 4 Weeks of Voluntary Aerobic Activity along with Interferon Beta on Serum Levels of Tumor Necrosis Factor Alpha (TNF-α) and Interleukin 10 (IL-10) in Mice Model of Multiple Sclerosis

Document Type : Original Article (s)

Authors

Department of Exercise Physiology, Payame Noor University, Alborz, Iran

Abstract

Background: The anti-inflammatory effects of exercise in chronic diseases are mediated by a decrease in pro-inflammatory cytokines and an increase in anti-inflammatory cytokines. The Aim of this study was to evaluate the effect of 4 weeks of voluntary aerobic activity along with interferon beta on the serum levels of tumor necrosis factor alfa (TNF-α) and interleukin 10 (IL-10) in mice model of multiple sclerosis.Methods: In this experimental study, a total number of 80 female mice were divided into eight groups. To induce of Experimental Autoimmune Encephalomyelitis (EAE), mixture of myelin oligodendrocyte glycoprotein (MOG) (35-55) was injected subcutaneously, and pertussis toxin was injected intraperitoneally. The interferon beta drug protocol in amount of 150 IU/g as well as the 4-week protocol of voluntary aerobic exercise with running wheel were administered to the treatment groups. Finally, blood samples were taken from the heart and data analysis was done using one-way ANOVA test.Findings: TNF-α levels in the MS+INF-β group significantly reduced compared to the MS + solvent group (P = 0.0010), and a significant increase was observed in the amount of IL-10 in comparison with the same groups (P = 0.01). But aerobic activity along with interferon beta-1 treatment, more effectively led to an increase in IL-10 of serum and a decrease in serum TNF-α in MS + INF-β + running wheel group compared to MS + solvent + running wheel group (compared to exercise or medication alone) (P = 0.001).Conclusion: Aerobic activity along with medication may possibly lead to clinical improvement and quality of life in patients with MS by controlling inflammatory factors.

Keywords

References

  1. Lassmann H. Multiple sclerosis pathology. Cold Spring Harb Perspect Med 2018; 8(3): 29358320.
  2. Sospedra M, Martin R. Immunology of multiple sclerosis. Semin Neurol 2016; 36(2): 115-27.
  3. Kierkegaard M, Lundberg IE, Olsson T, Johansson S, Ygberg S, Opava C, et al. High-intensity resistance training in multiple sclerosis - An exploratory study of effects on immune markers in blood and cerebrospinal fluid, and on mood, fatigue, health-related quality of life, muscle strength, walking and cognition. J Neurol Sci 2016; 362: 251-7.
  4. Skaper SD, Facci L, Zusso M, Giusti P. An inflammation-centric view of neurological disease: Beyond the neuron. Front Cell Neurosci 2018; 12: 72.
  5. Vatandoust M, Seyed Alhosseini SM, Nasirzadeh A, Jourabloo A. The effect of 4 weeks of aerobic activity on the ratio of Serum levels of tumor necrosis factor alpha, interleukin-10 and brain-derived neurotrophic factor in C57 rat brain tissue by induction of experimental autoimmune encephalomyelitis (EAE). J Shahid Sadoughi Univ Med Sci 2018; 26(7): 624-45. [In Persian].
  6. Martire S, Navone ND, Montarolo F, Perga S, Bertolotto A. A gene expression study denies the ability of 25 candidate biomarkers to predict the interferon-beta treatment response in multiple sclerosis patients. J Neuroimmunol 2016; 292: 34-9.
  7. Kim Y, Lai B, Mehta T, Thirumalai M, Padalabalanarayanan S, Rimmer JH, et al. Exercise training guidelines for multiple sclerosis, stroke, and parkinson disease: rapid review and synthesis. Am J Phys Med Rehabil 2019; 98(7): 613-21.
  8. Devasahayam AJ, Downer MB, Ploughman M. The effects of aerobic exercise on the recovery of walking ability and neuroplasticity in people with multiple sclerosis: A systematic review of animal and clinical studies. Mult Scler Int 2017; 2017: 4815958.
  9. White LJ, Castellano V. Exercise and brain health--implications for multiple sclerosis: Part 1--neuronal growth factors. Sports Med 2008; 38(2): 91-100.
  10. Abd El-Kader SM, Al-Shreef FM, Al-Jiffri OH. Impact of aerobic exercise versus resisted exercise on endothelial activation markers and inflammatory cytokines among elderly. Afr Health Sci 2019; 19(4): 2874-80.
  11. Mokhtarzade M, Ranjbar R, Majdinasab N, Patel D, Molanouri SM. Effect of aerobic interval training on serum IL-10, TNFalpha, and adipokines levels in women with multiple sclerosis: Possible relations with fatigue and quality of life. Endocrine 2017; 57(2): 262-71.
  12. Manzanares G, Brito-da-Silva G, Gandra PG. Voluntary wheel running: patterns and physiological effects in mice. Braz J Med Biol Res 2018; 52(1): e7830.
  13. Bartling B, Al-Robaiy S, Lehnich H, Binder L, Hiebl B, Simm A. Sex-related differences in the wheel-running activity of mice decline with increasing age. Exp Gerontol 2017; 87(Pt B): 139-47.
  14. Pryor Wm. Effects Of Exercise On Neuroprotection In Experimental Autoimmune Encephalomyelitis [PhD Thesis]. Athens, Ga: University Of Georgia; 2012.
  15. Andreasen AK, Stenager E, Dalgas U. The effect of exercise therapy on fatigue in multiple sclerosis. Mult Scler 2011; 17(9): 1041-54.
  16. Hosseini SM, fallah mohammadi Z, Feizi F. The effect of high-intensity resistance exercise period on the levels of interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α), and brain-derived neurotrophic factor (BDNF) in the brain of Lewis rats with experimental autoimmune encephalomyelitis. J Isfahan Med Sch 2017; 35(422): 270-7. [In Persian].
  17. White LJ, Castellano V, Mc Coy SC. Cytokine responses to resistance training in people with multiple sclerosis. J Sports Sci 2006; 24(8): 911-4.
  18. Rezaee S, Kahrizi S, Nabavi SM, Hedayati M. VEGF and TNF-α responses to acute and chronic aerobic exercise in the patients with multiple sclerosis. Asian J Sports Med 2020; 11(3): e98312.
  19. Pedersen BK, Steensberg A, Schjerling P. Exercise and interleukin-6. Curr Opin Hematol 2001; 8(3): 137-41.
  20. Cabral-Santos C, de Lima Junior EA, Fernandes IMDC, Pinto RZ, Rosa-Neto JC, Bishop NC, et al. Interleukin-10 responses from acute exercise in healthy subjects: A systematic review. J Cell Physiol 2019; 234(7): 9956-65.
  21. Cerqueira E, Marinho DA, Neiva HP, Lourenco O. Inflammatory effects of high and moderate intensity exercise-a systematic review. Front Physiol 2019; 10: 1550.
  22. Castellano V, Patel DI, White LJ. Cytokine responses to acute and chronic exercise in multiple sclerosis. J Appl Physiol (1985) 2008; 104(6): 1697-702.
  23. Morgan JA, Singhal G, Corrigan F, Jaehne EJ, Jawahar MC, Baune BT. TNF signalling via the TNF receptors mediates the effects of exercise on cognition-like behaviours. Behav Brain Res 2018; 353: 74-82.
  24. Koh Y, Park KS. Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals. J Exerc Rehabil 2017; 13(4): 472-6.
  25. Negaresh R, Motl RW, Mokhtarzade M, Dalgas U, Patel D, Shamsi MM, et al. Effects of exercise training on cytokines and adipokines in multiple Sclerosis: A systematic review. Mult Scler Relat Disord 2018; 24: 91-100.
  26. Paolucci EM, Loukov D, Bowdish DME, Heisz JJ. Exercise reduces depression and inflammation but intensity matters. Biol Psychol 2018; 133: 79-84.
  27. Bashiri H, Enayati M, Bashiri A, Salari AA. Swimming exercise improves cognitive and behavioral disorders in male NMRI mice with sporadic Alzheimer-like disease. Physiol Behav 2020; 223: 113003.
  28. Suzuki K. Cytokine response to exercise and its modulation. Antioxidants (Basel) 2018; 7(1): 17.
  29. Guo LY, Lozinski B, Yong VW. Exercise in multiple sclerosis and its models: Focus on the central nervous system outcomes. J Neurosci Res 2020; 98(3): 509-23.
  30. Trenova AG, Miteva LD, Stanilova SA. Association between TNFA, IL10 and IL18 promoter gene variants and cognitive functions in patients with relapsing-remitting multiple sclerosis. J Neuroimmunol 2020; 347: 577357.
Journal of Isfahan Medical School
Volume 39, Issue 651 - Serial Number 651
November and December 2022
Pages 906-913

Files

History

  • Receive Date: 05 April 2021
  • Revise Date: 02 October 2022
  • Accept Date: 06 April 2022

Share

How to cite

Statistics