Separate and Concomitant Effect of Swimming Exercise and Quercetin Supplementation on Rat’s Knee Osteoarthritis

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran

2 PhD Student, Department of Sport Medicine, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

3 Associate Professor, Department of Sport Medicine, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

4 Associate Professor, Department of Sport Physiology, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

5 PhD Student, Department of Sport Traumatology, School of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran

Abstract

Background: Osteoarthritis is the most prevalent musculoskeletal disorder leading to chronic pain and severe disability and its prevalence is predicted to raise significantly in the future as the population ages.Methods: Thirty male Wistar rats (weight of 173 ± 1 g, age of 8 weeks) were randomly divided into 5 equal experimental groups: 1. Intact control, 2. Osteoarthritis, 3. Quercetin 4. Swimming, and 5. Quercetin + Swimming. Intra-articular injection of 50 µl of monosodium iodoacetate (MIA) was applied on right knee of rats; similarly, saline was injected in left knees. The used training program was swimming in moderate intensity for 28 days. After the completion of the protocol, rats were killed and both knees of the animals were assessed histopathologically. One-way analysis of variances (ANOVA) and post-hoc Tukey tests were used for statistical analysis, at the significant level of P < 0.05.Findings: Results indicated a significant difference between quercetin, swimming and quervetin + swimming groups with the osteoarthritis group (P = 0.001).Conclusion: Regarding results of the study, it can be concluded that swimming exercise at moderate intensity and quercetin supplementation, either alone or in combination, have a positive effect on improvement of osteoarthritis symptoms of rat’s knee. But, it seems that separate effect of quercetin supplementation and its combination with swimming exercise does not excel the effect of swimming exercise alone.

Keywords

References

  1. Hinman RS, Heywood SE, Day AR. Aquatic physical therapy for hip and knee osteoarthritis: results of a single-blind randomized controlled trial. Phys Ther 2007; 87(1): 32-43.
  2. Bayat N, Gharibdoost F, Nasiri Afshar F, Zamani B. Clinical and radiological analysis of OA patient reffereing to Baghiatallah hospital during 2003-2004. Feyz 2006; 10(1): 42-5. [In Persian].
  3. Hadipour M, Mozaffari R. Protective effects of nutrients on articular cartilage of rats and its histological assessment. Daneshvar Med 2008; 15(75): 87-94. [In Persian].
  4. Gupta S, Hawker GA, Laporte A, Croxford R, Coyte PC. The economic burden of disabling hip and knee osteoarthritis (OA) from the perspective of individuals living with this condition. Rheumatology (Oxford) 2005; 44(12): 1531-7.
  5. Puett DW, Griffin MR. Published trials of nonmedicinal and noninvasive therapies for hip and knee osteoarthritis. Ann Intern Med 1994; 121(2): 133-40.
  6. Lay E, Samiric T, Handley CJ, Ilic MZ. Short- and long-term exposure of articular cartilage to curcumin or quercetin inhibits aggrecan loss. J Nutr Biochem 2012; 23(2): 106-12.
  7. Panico AM, Cardile V, Garufi F, Puglia C, Bonina F, Ronsisvalle G. Protective effect of Capparis spinosa on chondrocytes. Life Sci 2005; 77(20): 2479-88.
  8. Zhang W, Moskowitz RW, Nuki G, Abramson S, Altman RD, Arden N, et al. OARSI recommendations for the management of hip and knee osteoarthritis, Part II: OARSI evidence-based, expert consensus guidelines. Osteoarthritis Cartilage 2008; 16(2): 137-62.
  9. Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Farmaco 2001; 56(9): 683-7.
  10. Clyman B. Exercise in the treatment of osteoarthritis. Curr Rheumatol Rep 2001; 3(6): 520-3.
  11. Bennell KL, Hinman RS. A review of the clinical evidence for exercise in osteoarthritis of the hip and knee. J Sci Med Sport 2011; 14(1): 4-9.
  12. Blagojevic M, Jinks C, Jeffery A, Jordan KP. Risk factors for onset of osteoarthritis of the knee in older adults: a systematic review and meta-analysis. Osteoarthritis Cartilage 2010; 18(1): 24-33.
  13. Andersson ML, Thorstensson CA, Roos EM, Petersson IF, Heinegard D, Saxne T. Serum levels of cartilage oligomeric matrix protein (COMP) increase temporarily after physical exercise in patients with knee osteoarthritis. BMC Musculoskelet Disord 2006; 7: 98.
  14. Roddy E, Zhang W, Doherty M, Arden NK, Barlow J, Birrell F, et al. Evidence-based recommendations for the role of exercise in the management of osteoarthritis of the hip or knee--the MOVE consensus. Rheumatology (Oxford) 2005; 44(1): 67-73.
  15. Ng NT, Heesch KC, Brown WJ. Efficacy of a progressive walking program and glucosamine sulphate supplementation on osteoarthritic symptoms of the hip and knee: a feasibility trial. Arthritis Res Ther 2010; 12(1): R25.
  16. Messier SP, Mihalko S, Loeser RF, Legault C, Jolla J, Pfruender J, et al. Glucosamine/chondroitin combined with exercise for the treatment of knee osteoarthritis: a preliminary study. Osteoarthritis Cartilage 2007; 15(11): 1256-66.
  17. Galois L, Etienne S, Grossin L, Cournil C, Pinzano A, Netter P, et al. Moderate-impact exercise is associated with decreased severity of experimental osteoarthritis in rats. Rheumatology (Oxford) 2003; 42(5): 692-3.
  18. Galois L, Etienne S, Grossin L, Watrin-Pinzano A, Cournil-Henrionnet C, Loeuille D, et al. Dose-response relationship for exercise on severity of experimental osteoarthritis in rats: a pilot study. Osteoarthritis Cartilage 2004; 12(10): 779-86.
  19. Cifuentes DJ, Rocha LG, Silva LA, Brito AC, Rueff-Barroso CR, Porto LC, et al. Decrease in oxidative stress and histological changes induced by physical exercise calibrated in rats with osteoarthritis induced by monosodium iodoacetate. Osteoarthritis Cartilage 2010; 18(8): 1088-95.
  20. Lee YJ, Park JA, Yang SH, Kim KY, Kim BK, Lee EY, et al. Evaluation of osteoarthritis induced by treadmill-running exercise using the modified Mankin and the new OARSI assessment system. Rheumatol Int 2011; 31(12): 1571-6.
  21. Vignon E, Valat JP, Rossignol M, Avouac B, Rozenberg S, Thoumie P, et al. Osteoarthritis of the knee and hip and activity: a systematic international review and synthesis (OASIS). Joint Bone Spine 2006; 73(4): 442-55.
  22. Fisher NM, Dolan DM, Brenner C, Pendergast DR. Quantitative effects of a water exercise program on functional and physiological capacity in subjects with knee osteoarthritis: a pilot study. Sport Sci Health 2004; 1(1): 17-24.
  23. Lohmander LS, Ostenberg A, Englund M, Roos H. High prevalence of knee osteoarthritis, pain, and functional limitations in female soccer players twelve years after anterior cruciate ligament injury. Arthritis Rheum 2004; 50(10): 3145-52.
  24. Roe J, Pinczewski LA, Russell VJ, Salmon LJ, Kawamata T, Chew M. A 7-year follow-up of patellar tendon and hamstring tendon grafts for arthroscopic anterior cruciate ligament reconstruction: differences and similarities. Am J Sports Med 2005; 33(9): 1337-45.
  25. Cohen M, Amaro JT, Ejnisman B, Carvalho RT, Nakano KK, Peccin MS, et al. Anterior cruciate ligament reconstruction after 10 to 15 years: association between meniscectomy and osteoarthrosis. Arthroscopy 2007; 23(6): 629-34.
  26. Jarvela T, Paakkala T, Kannus P, Jarvinen M. The incidence of patellofemoral osteoarthritis and associated findings 7 years after anterior cruciate ligament reconstruction with a bone-patellar tendon-bone autograft. Am J Sports Med 2001; 29(1): 18-24.
  27. Neuman P, Englund M, Kostogiannis I, Friden T, Roos H, Dahlberg LE. Prevalence of tibiofemoral osteoarthritis 15 years after nonoperative treatment of anterior cruciate ligament injury: a prospective cohort study. Am J Sports Med 2008; 36(9): 1717-25.
  28. Bitton R. The economic burden of osteoarthritis. Am J Manag Care 2009; 15(8 Suppl): S230-5.
  29. National Institute of Arthritis and Musculoskeletal and Skin Diseases. Handout on health: osteoarthritis [Online]. [cited 2003 Aug]; Available from: URL:
  30. http://www.niams.nih.gov/Health_Info/Osteoarthritis/
  31. Manek NJ, Lane NE. Osteoarthritis: current concepts in diagnosis and management. Am Fam Physician 2000; 61(6): 1795-804.
  32. Lee YC, Shmerling RH. The benefit of nonpharmacologic therapy to treat symptomatic osteoarthritis. Curr Rheumatol Rep 2008; 10(1): 5-10.
  33. Haihong SHEN, Changjie Z. The interaction of static magnetic field and swimming exercises on articular cartilage of rat's knee osteoarthritis. Chinese J Rehabil Med 2009; (24)8: 702-6.
  34. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicol Pathol 2003; 31(6): 619-24.
  35. Kobayashi K, Imaizumi R, Sumichika H, Tanaka H, Goda M, Fukunari A, et al. Sodium iodoacetate-induced experimental osteoarthritis and associated pain model in rats. J Vet Med Sci 2003; 65(11): 1195-9.
  36. Gerwin N, Bendele AM, Glasson S, Carlson CS. The OARSI histopathology initiative - recommendations for histological assessments of osteoarthritis in the rat. Osteoarthritis Cartilage 2010; 18(Suppl 3): S24-S34.
  37. Huang J, Zhu M, Tao Y, Wang S, Chen J, Sun W, et al. Therapeutic properties of quercetin on monosodium urate crystal-induced inflammation in rat. J Pharm Pharmacol 2012; 64(8): 1119-27.
  38. Manach C, Williamson G, Morand C, Scalbert A, Remesy C. Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr 2005; 81(1 Suppl): 230S-42S.
  39. Jackson JK, Higo T, Hunter WL, Burt HM. The antioxidants curcumin and quercetin inhibit inflammatory processes associated with arthritis. Inflamm Res 2006; 55(4): 168-75.
  40. Kandere-Grzybowska K, Kempuraj D, Cao J, Cetrulo CL, Theoharides TC. Regulation of IL-1-induced selective IL-6 release from human mast cells and inhibition by quercetin. Br J Pharmacol 2006; 148(2): 208-15.
  41. Min YD, Choi CH, Bark H, Son HY, Park HH, Lee S, et al. Quercetin inhibits expression of inflammatory cytokines through attenuation of NF-kappaB and p38 MAPK in HMC-1 human mast cell line. Inflamm Res 2007; 56(5): 210-5.
  42. Tiku ML, Gupta S, Deshmukh DR. Aggrecan degradation in chondrocytes is mediated by reactive oxygen species and protected by antioxidants. Free Radic Res 1999; 30(5): 395-405.
  43. Kleemann R, Verschuren L, Morrison M, Zadelaar S, van Erk MJ, Wielinga PY, et al. Anti-inflammatory, anti-proliferative and anti-atherosclerotic effects of quercetin in human in vitro and in vivo models. Atherosclerosis 2011; 218(1): 44-52.
  44. Poole A, Howell D. Etiopathogenesis of osteoarthritis. In: Moskowitz RW, editor. Osteoarthritis: diagnosis and medical/surgical management. Philadelphia, PA: Lippincott Williams and Wilkins; 2001. p. 27-49.
  45. Felson DT, Lawrence RC, Dieppe PA, Hirsch R, Helmick CG, Jordan JM, et al. Osteoarthritis: new insights. Part 1: the disease and its risk factors. Ann Intern Med 2000; 133(8): 635-46.
  46. Villemure I, Chung MA, Seck CS, Kimm MH, Matyas JR, Duncan NA. Static compressive loading reduces the mRNA expression of type II and X collagen in rat growth-plate chondrocytes during postnatal growth. Connect Tissue Res 2005; 46(4-5): 211-9.
  47. Fanning PJ, Emkey G, Smith RJ, Grodzinsky AJ, Szasz N, Trippel SB. Mechanical regulation of mitogen-activated protein kinase signaling in articular cartilage. J Biol Chem 2003; 278(51): 50940-8.
  48. Chowdhury TT, Akanji OO, Salter DM, Bader DL, Lee DA. Dynamic compression influences interleukin-1beta-induced nitric oxide and prostaglandin E2 release by articular chondrocytes via alterations in iNOS and COX-2 expression. Biorheology 2008; 45(3-4): 257-74.
Journal of Isfahan Medical School
Volume 32, Issue 288 - Serial Number 288
May and June 2014
Pages 805-817

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History

  • Receive Date: 04 November 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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