Prognostic Importance of Polymorphisms in DNA-Repair Gene (XRCC1 Arg399Gln) in Patients with Lung Cancer in Fars Province, Iran

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Cellular and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran

2 Assistant Professor, Department of Cell and Molecular Sciences, School of Biological Sciences, Kharazmi University, Tehran, Iran

3 Professor, Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Background: Genetic polymorphisms in DNA-repair genes may increase the risk of developing cancer due to reducing the DNA-repair capacity. XRCC1 is one of the important genes in DNA repair. This study was designed to examine the polymorphisms associated with XRCC1 Arg399Gln and to investigate its role as susceptibility marker for non-small cell lung cancer (NSCLC) in population of Fars province, Iran.Methods: In this case-control study, extracted DNA from 100 healthy controls and 100 patient of lung cancer were used to examine the role of XCRR1 Arg399Gln polymorphism in context of non-small cell lung cancer in population of Fars province in Iran. Amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used for determining of individuals’ genotyping.Findings: Our data showed a strong association between Gln/Gln (A/A) genotype and risk of developing non-small cell lung cancer in men. Moreover, men with at least one A allele (AA+ AG) showed reduced risk of developing non-small cell lung cancer. No such an associations were found in subgroups of women or when samples were divided based on their ages.Conclusion: According to our results, although there was no significant association between XRCC1 Age399Gln polymorphism and developing of lung cancer in population, men with Gln/Gln genotype were in high risk of developing non-small cell lung cancer in Fars province, Iran. Therefore, Gln/Gln polymorphism could be used as a biomarker for screening men at high risk of lung cancer.

Keywords

References

  1. Dela Cruz CS, Tanoue LT, Matthay RA. Lung cancer: epidemiology, etiology, and prevention. Clin Chest Med 2011; 32(4): 605-44.
  2. Hosseini M, Naghan PA, Karimi S, SeyedAlinaghi S, Bahadori M, Khodadad K, et al. Environmental risk factors for lung cancer in Iran: a case-control study. Int J Epidemiol 2009; 38(4): 989-96.
  3. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin 2011; 61(2): 69-90.
  4. Bernstein C, Bernstein H, Payne CM, Garewal H. DNA repair/pro-apoptotic dual-role proteins in five major DNA repair pathways: fail-safe protection against carcinogenesis. Mutat Res 2002; 511(2):
  5. -78.
  6. Vidal AE, Boiteux S, Hickson ID, Radicella JP. XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions. EMBO J 2001; 20(22): 6530-9.
  7. Lu AL, Li X, Gu Y, Wright PM, Chang DY. Repair of oxidative DNA damage: mechanisms and functions. Cell Biochem Biophys 2001; 35(2): 141-70.
  8. Op het Veld CW, Jansen J, Zdzienicka MZ, Vrieling H, van Zeeland AA. Methyl methanesulfonate-induced hprt mutation spectra in the Chinese hamster cell line CHO9 and its xrcc1-deficient derivative EM-C11. Mutat Res 1998; 398(1-2): 83-92.
  9. Thompson LH, Brookman KW, Dillehay LE, Carrano AV, Mazrimas JA, Mooney CL, et al. A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange. Mutat Res 1982; 95(2-3): 427-40.
  10. Zdzienicka MZ, van der Schans GP, Natarajan AT, Thompson LH, Neuteboom I, Simons JW. A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple alkylating agents and a very high level of sister chromatid exchanges. Mutagenesis 1992; 7(4): 265-9.
  11. Almeida KH, Sobol RW. A unified view of base excision repair: lesion-dependent protein complexes regulated by post-translational modification. DNA Repair (Amst ) 2007; 6(6): 695-711.
  12. Xing D, Qi J, Miao X, Lu W, Tan W, Lin D. Polymorphisms of DNA repair genes XRCC1 and XPD and their associations with risk of esophageal squamous cell carcinoma in a Chinese population. Int J Cancer 2002; 100(5): 600-5.
  13. Duell EJ, Holly EA, Bracci PM, Wiencke JK, Kelsey KT. A population-based study of the Arg399Gln polymorphism in X-ray repair cross- complementing group 1 (XRCC1) and risk of pancreatic adenocarcinoma. Cancer Res 2002; 62(16): 4630-6.
  14. Chacko P, Rajan B, Joseph T, Mathew BS, Pillai MR. Polymorphisms in DNA repair gene XRCC1 and increased genetic susceptibility to breast cancer. Breast Cancer Res Treat 2005; 89(1): 15-21.
  15. Lamerdin JE, Montgomery MA, Stilwagen SA, Scheidecker LK, Tebbs RS, Brookman KW, et al. Genomic sequence comparison of the human and mouse XRCC1 DNA repair gene regions. Genomics 1995; 25(2): 547-54.
  16. Cao Y, Miao XP, Huang MY, Deng L, Hu LF, Ernberg I, et al. Polymorphisms of XRCC1 genes and risk of nasopharyngeal carcinoma in the Cantonese population. BMC Cancer 2006; 6: 167.
  17. Shen H, Xu Y, Qian Y, Yu R, Qin Y, Zhou L, et al. Polymorphisms of the DNA repair gene XRCC1 and risk of gastric cancer in a Chinese population. Int J Cancer 2000; 88(4): 601-6.
  18. Chen S, Tang D, Xue K, Xu L, Ma G, Hsu Y, et al. DNA repair gene XRCC1 and XPD polymorphisms and risk of lung cancer in a Chinese population. Carcinogenesis 2002; 23(8): 1321-5.
  19. Zhou W, Liu G, Miller DP, Thurston SW, Xu LL, Wain JC, et al. Polymorphisms in the DNA repair genes XRCC1 and ERCC2, smoking, and lung cancer risk. Cancer Epidemiol Biomarkers Prev 2003; 12(4): 359-65.
  20. Li C, Hu Z, Lu J, Liu Z, Wang LE, El-Naggar AK, et al. Genetic polymorphisms in DNA base-excision repair genes ADPRT, XRCC1, and APE1 and the risk of squamous cell carcinoma of the head and neck. Cancer 2007; 110(4): 867-75.
  21. Al Mutairi FM, Alanazi M, Shalaby M, Alabdulkarim HA, Pathan AA, Parine NR. Association of XRCC1 gene polymorphisms with breast cancer susceptibility in Saudi patients. Asian Pac J Cancer Prev 2013; 14(6): 3809-13.
  22. Khan NP, Pandith AA, Yousuf A, Khan NS, Khan MS, Bhat IA, et al. The XRCC1 Arg399Gln gene polymorphism and risk of colorectal cancer: a study in Kashmir. Asian Pac J Cancer Prev 2013; 14(11): 6779-82.
  23. Halim NH, Chong ET, Goh LP, Chuah JA, See EU, Chua KH, et al. Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo. Asian Pac J Cancer Prev 2016; 17(4): 1925-31.
  24. Natukula K, Jamil K, Pingali UR, Attili VS, Madireddy UR. The codon 399 Arg/Gln XRCC1 polymorphism is associated with lung cancer in Indians. Asian Pac J Cancer Prev 2013; 14(9): 5275-9.
  25. Ke HG, Li J, Shen Y, You QS, Yan Y, Dong HX, et al. Prognostic significance of GSTP1, XRCC1 and XRCC3 polymorphisms in non-small cell lung cancer patients. Asian Pac J Cancer Prev 2012; 13(9): 4413-6.
  26. Improta G, Sgambato A, Bianchino G, Zupa A, Grieco V, La TG, et al. Polymorphisms of the DNA repair genes XRCC1 and XRCC3 and risk of lung and colorectal cancer: a case-control study in a Southern Italian population. Anticancer Res 2008; 28(5B): 2941-6.
  27. Lopez-Cima MF, Gonzalez-Arriaga P, Garcia-Castro L, Pascual T, Marron MG, Puente XS, et al. Polymorphisms in XPC, XPD, XRCC1, and XRCC3 DNA repair genes and lung cancer risk in a population of northern Spain. BMC Cancer 2007; 7: 162.
  28. Jemal A, Center MM, Ward E. The convergence of lung cancer rates between blacks and whites under the age of 40, United States. Cancer Epidemiol Biomarkers Prev 2009; 18(12): 3349-52.
Journal of Isfahan Medical School
Volume 34, Issue 403 - Serial Number 403
September and October 2016
Pages 1244-1250

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History

  • Receive Date: 20 August 2016
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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