Molecular Study of the Prevalence of Exotoxin A and Alginate Gene in Pseudomonas Aeruginosa Isolates in Burn Wounds Samples

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Department of Microbiology, School of Science, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran

3 Associate Professor, Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

4 Associate Professor, Department of Infectious Disease, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

5 Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

Background: Pseudomonas aeruginosa is one of the major nosocomial pathogens with a vast antibiotic resistance and great ability in adaptation with environment. The purpose of this study was to evaluate the antibiotic resistance and bacterial detection using Exotoxin A (ETA) and algD genes in patients with burns wound infection.Methods: In this study, 188 samples were evaluated with standard bacteriological methods. After antibiotic resistance evaluation with disc diffusion method (Kirby-Bauer), specific primers for detection of algD and ETA genes among bacterial isolate were used. All data were analyzed using SPSS software.Findings: From 188 taken samples, 91 isolates were Pseudomonas aeruginosa. The antibiotic resistance rate for cefotaxime, cefepime, amikacin, ceftazidime, gentamicin, piperacillin, ciprofloxacin and imipenem were 80 (87.9%), 77 (84.6%), 69 (75.8%), 67 (73.6%), 66 (72.6%), 63 (69.2%), 62 (68.2%) and 53 (58.2%), respectively. The prevalence of algD and ETA genes were 88 (96.7%) and 79 (87.0%), respectively.Conclusion: The high prevalence of algD and ETA genes among burns wound infections demonstrates the increase in pathogenesis of Pseudomonas aeruginosa and problems with burn wound infection treatment. Therefore, accurate and rapid diagnosis of resistant Pseudomonas aeruginosa can be useful in taking proper strategy for prevention and treatment of such infections.

Keywords

References

  1. Akya A, Salimi A, Nomanpour B, Ahmadi K. Prevalence and clonal dissemination of metallo-beta-lactamase-producing Pseudomonas aeruginosa in Kermanshah. Jundishapur J Microbiol 2015; 8(7): e20980.
  2. Driscoll JA, Brody SL, Kollef MH. The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs 2007; 67(3): 351-68.
  3. Alhazmi A. Pseudomonas aeruginosa – pathogenesis and pathogenic mechanisms. International Journal of Biology 2015; 7(2): 44-67.
  4. Mendiratta DK, Deotale V, Narang P. Metallo-beta-lactamase producing Pseudomonas aeruginosa in a hospital from a rural area. Indian J Med Res 2005; 121(5): 701-3.
  5. Al-Daraghi WA, Abdullah ZH. Detection of exotoxin a gene in Pseudomonas aeruginosa from clinical and environmental samples. Journal of Al-Nahrain University 2013; 16(2): 167-72.
  6. Xiao X, Zhang J, Gong J, Pan Y, Yu Y, Yang X, et al. Rapid detection of Pseudomonas aernginosa by the fluorescence quantitative TaqMan PCR assay targetting ETA gene. Sheng Wu Gong Cheng Xue Bao 2008; 24(4): 581-5. [In Chinese].
  7. Xu J, Moore JE, Murphy PG, Millar BC, Elborn JS. Early detection of Pseudomonas aeruginosa--comparison of conventional versus molecular (PCR) detection directly from adult patients with cystic fibrosis (CF). Ann Clin Microbiol Antimicrob 2004; 3: 21.
  8. Hafiane A, Ravaoarinoro M. Characterization of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients by different typing methods. Pathol Biol (Paris) 2011; 59(5): e109-e114.
  9. Ramsey DM, Wozniak DJ. Understanding the control of Pseudomonas aeruginosa alginate synthesis and the prospects for management of chronic infections in cystic fibrosis. Mol Microbiol 2005; 56(2): 309-22.
  10. Amirmozafari N, Fallah Mehrabadi J, Isazadieh K, Habibi A. Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals. Iran J Med Microbiol 2015; 8(4): 36-43. [In Persian].
  11. Akhavan Tafti F, Zandi H, Vakli M, Mousavi SM, Zarei M. Frequency of β-lactamase and metallo-β-lactamase in Pseudomonas aeruginosa strains isolated from burn wounds in Yazd burn hospital during 2011-2012. Feyz 2014; 18(2): 167-74. [In Persian].
  12. Wang H, Tu F, Gui Z, Lu X, Chu W. Antibiotic resistance profiles and quorum sensing-dependent virulence factors in clinical isolates of pseudomonas aeruginosa. Indian J Microbiol 2013; 53(2): 163-7.
  13. Tramper-Stranders GA, van der Ent CK, Wolfs TF. Detection of Pseudomonas aeruginosa in patients with cystic fibrosis. J Cyst Fibros 2005; 4(Suppl 2): 37-43.
  14. Clinical Laboratory Standards Institute. M100-S22. Performance standards for antimicrobial susceptibility testing; twenty-second international supplement. Wayne, PA: Clinical Laboratory Standards Institute; 2012. p. 50-1.
  15. Valadbeigi H, Sadeghifard N, Rafiei Tabatabaei R, Maleki A. A study on the frequency of toxin A, alginate genes, and of clinical Pseudomonas aeroginosa strains. J Ilam Univ Med Sci 2012; 20(1): 58-64. [In Persian].
  16. Aghaei SS, Javadi A, Sharifi Y, Morovvati A. Detection of exotoxin A, Y, T, U, S genes of Pseudomonas aeruginosa isolates resistant to third-generation cephalosporins in clinical samples of hospitalized patients in hospitals of Qom city, Iran. Qom Univ Med Sci J 2016; 10(1): 48-55. [In Persian].
  17. Fazeli N, Momtaz H. Virulence gene profiles of multidrug-resistant Pseudomonas aeruginosa isolated from Iranian hospital infections. Iran Red Crescent Med J 2014; 16(10): e15722.
  18. Yousefi Mashouf R, Esmaeili R, Alikhani MY, Ghanbari M. Evaluation of exotoxin A gene and frequency of polymerase chain reaction sensitivity in detection of pseudomonas aeruginosa isolated from burn patients. Tehran Univ Med J 2014; 72(3): 167-73. [In Persian].
  19. Zavascki AP, Barth AL, Fernandes JF, Moro AL, Goncalves AL, Goldani LZ. Reappraisal of Pseudomonas aeruginosa hospital-acquired pneumonia mortality in the era of metallo-beta-lactamase-mediated multidrug resistance: a prospective observational study. Crit Care 2006; 10(4): R114.
  20. Ranjan KP, Ranjan N, Bansal SK, Arora DR. Prevalence of Pseudomonas aeruginosa in post-operative wound infection in a referral hospital in Haryana, India. J Lab Physicians 2010; 2(2): 74-7.
  21. Abdi-Ali A, Nikbin V, Feizabadi MM, Gharavi S, Fallahi Z. Study of Plasmid Profile and Antibiotic Resistance in Hospital Pseudomonas aeruginosa. Iranian Journal of Biology 2005; 18(2): 141-9.
  22. Fazeli H, Fatahi Bafghi M, Faghri M, Akbari R. Molecular Study Of PER and VEB genes is multidrug resistant Pseudomonas Aeroginosa isolated from clinical specimens in Isfahan/Iran and their antibiotic resistance patterns. J Kerman Univ Med Sci 2012; 19(4): 345-53. [In Persian].
  23. Mirsalehian A, Akbari Nakhjavani F, Bahador A, Jabal ameli F, Bigverdi R, Goli H. Prevalence of MBL-producing Pseudomonas aeruginosa isolated from burn patients. Tehran Univ Med J 2011; 68(10): 563-9. [In Persian].
  24. Imani Foolad A, Hosainzadeh M, Mousavi S F. Association between exotoxin A (exo-A) gene and antibiotic resistance pattern with biofilm formation in Pseudomonas aeruginosa. J Ardabil Univ Med Sci 2011; 11(1): 7-13. [In Persian].
  25. Amini B, Kamali M, Zarei Mahmod abadi A, Mortazavi Y, Ebrahim habibi A, Bayat E, et al. Cloning of catalytic domain of exotoxin A from Pseudomonas aeruginosa. J Zanjan Univ Med Sci 2010; 18(71): 24-33. [In Persian].
  26. Pourzereshki N, Naserpour Farivar T, Peymani A. Presence of alginate among multidrug resistant Pseudomonas aeruginosa isolated from clinical samples in Qazvin and Tehran hospitals. J Clin Res Paramed Sci 2015; 3(4): 257-63. [In Persian].
  27. Rashno TS, Khansarinejad B, Abtahi H, Najafimosleh M, Ghaznavi-Rad E. Detection of algD, oprL and exoA Genes by new specific primers as an efficient, rapid and accurate procedure for direct diagnosis of Pseudomonas aeruginosa strains in clinical samples. Jundishapur J Microbiol 2014; 7(10): e13583.
  28. Stehling EG, Silveira WD, Leite DS. Study of biological characteristics of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis and from patients with extra-pulmonary infections. Braz J Infect Dis 2008; 12(1): 86-8.
Journal of Isfahan Medical School
Volume 34, Issue 412 - Serial Number 412
November and December 2017
Pages 1537-1543

Files

History

  • Receive Date: 28 August 2016
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics