Prevalence of CTX-M Genes in Escherichia Coli Strains in Outpatient and Inpatient Cases with Urinary Tract Infections in Isfahan, Iran

Document Type : Original Article (s)

Authors

1 Department of Genetics and Biotechnology, School of Sciences, Payame Noor University, Tehran, Iran

2 Assistant Professor, Department of Genetics and Biotechnology, School of Sciences, Payame Noor University, Tehran, Iran

3 Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan Iran

Abstract

Background: Extended-spectrum beta-lactamase of CTX-M-type is considered as an important mechanism resistant to beta-lactam drugs in the gram-negative pathogens. In this study, the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) as well as the frequency of CTX-M genes from urinary tract infections in inpatients and outpatients caused by Escherichia coli in selected health centers in Isfahan, Iran, was studied in 2013.Methods: The study was carried out on 120 E. coli isolates from inpatients and outpatients with urinary tract infection. The susceptibility of isolates was tested using disc diffusion method. The ESBL-producer strains were confirmed using combined discs method. Finally, the blaCTX-M gene was determined in ESBL-producer isolates using polymerase chain reaction (PCR) method. To identify the strains of E. coli producing CTX-M genes, PCR method was used. The collected data were entered and analyzed in WHONET 5.6 and Excel softwares.Findings: From 120 strains of E. coli, 12 outpatient (20.0%) and 33 inpatient (55.0%) isolates were ESBL-producer. 84.4% and 60.1% of outpatient isolates and 94.9% and 84.8% of inpatient isolates showed resistance to ampicillin and co-trimoxazole, respectively. PCR analysis revealed that 30 isolates had CTX-M gene.Conclusion: The production of ESBL, in particular in isolates of inpatients, is a big threat for use of the broad-spectrum cephalosporins. Given the presence of CTX-M gene in the high proportion of the isolates, more molecular and epidemiological studies on pathogenic bacteria in health centers is required to be able to have a common plan to control bacterial resistance.

Keywords

References

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Journal of Isfahan Medical School
Volume 33, Issue 360 - Serial Number 360
September and October 2016
Pages 2019-2025

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History

  • Receive Date: 13 August 2015
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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