The Effect of Betaine on Cerebellar Histological Alterations, Balance, Motor Function on Male Rats, an Experimental Model of Multiple Sclerosis

Document Type : Original Article (s)

Authors

1 PhD Candidate of Comparative Histology, Division of Basic Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Assistant Professor, Department of Basic Sciences, Division of Histology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Associate Professor, Department of Basic Sciences, Division of Biochemistry and Molecular Biology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Associate Professor, Department of Basic Sciences, Division of Physiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

5 Assistant Professor, Department of Basic Sciences, Division of Anatomy and Embryology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Background: One of the chronic inflammatory diseases of the central nervous system is multiple sclerosis (MS). Betaine has anti-inflammatory and neuroprotective effects. The present study was conducted with the aim of investigating the effect of betaine on tissue changes of the cerebellum and motor activity in the experimental model of MS.
Methods: In this experimental research, 20 adult male rats (12-week-old) were divided into control, Ms, Ms+ betaine and betaine. To create the MS model, animals were fed food containing 0.5% cuprizone for 12 weeks. For treatment, betaine was given at a dose of 1% in drinking water for the last 6 weeks. At the end of period, in order to measure balance and motor coordination, rotarod, open field, and inverted grid tests were performed, and the cerebellum of the animals was studied in terms of histological alterations.
Findings: Motor activities and maintaining balance in the MS group showed a significant decrease compared to control group, while treatment with betaine improved these symptoms. In the histological studies, tissue changes in Purkinje cells, such as a decrease in the number, condensation and pyknosis of the nucleus, a decrease in the diameter of the cell body and the nucleus of these cells were seen in the MS group. While the MS group receiving betaine, the changes were clearly improved and the Purkinje cells were able to maintain their number and shape.
Conclusion: The betaine can be considered as an effective biomolecule in the process of nerve regeneration and improvement of motor behaviors in patients with MS.

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References

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Journal of Isfahan Medical School
Volume 41, Issue 734
2nd Week, November
November and December 2023
Pages 773-779

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History

  • Receive Date: 16 July 2023
  • Revise Date: 20 September 2023
  • Accept Date: 20 September 2023

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