Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Biomedical Engineering, School of Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Associate Professor, Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Iran Polymer and Petrochemical Institute AND Department of Biomedical Engineering, School of Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Assistant Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Scaffold, an essential element of tissue engineering, should provide proper physical and chemical properties and evolve suitable cell behavior for tissue regeneration.Methods: Polycaprolactone/gelatin-based nanocomposite scaffolds containing nano-hydroxyapatite and vitamin D3 was fabricated using the electrospinning method. To obtain suitable properties, solution and process parameters were optimized. Fiber morphology and MG-63 cells were cultured; chemical interactions between molecules forming scaffold and the amount of mineral deposition were determined via scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR) and alizarin red staining, respectively.Findings: The mean diameter of PCL/Gel/nHA/Vit D3 nanofibers was about 531 nm. The culture of osteoblast cells on the scaffolds showed that the addition of Vit D3 to PCL/Gel/nHA scaffold caused further attachment and proliferation of the cells. In addition, stained mineral deposits scaffolds with alizarin red staining showed that the amount of mineralized deposits was significantly higher in PCL/Gel/nHA/Vit D3 scaffold than other scaffolds (P < 0.05).Conclusion: Superior properties of nano-hydroxyapatite and vitamin D3 blended in polycaprolactone/gelatin-based scaffold were confirmed. The observations also revealed that the composite scaffold could be a good candidate for bone tissue engineering.

Keywords

References

  1. Vasita R, Katti DS. Nanofibers and their applications in tissue engineering. Int J Nanomedicine 2006; 1(1): 15-30.
  2. Khorshidi S, Solouk A, Mirzadeh H, Mazinani S, Lagaron JM, Sharifi S, et al. A review of key challenges of electrospun scaffolds for tissue-engineering applications. J Tissue Eng Regen Med 2016; 10(9): 715-38.
  3. Vatankhah E, Semnani D, Prabhakaran MP, Tadayon M, Razavi S, Ramakrishna S. Artificial neural network for modeling the elastic modulus of electrospun polycaprolactone/gelatin scaffolds. Acta Biomater 2014; 10(2): 709-21.
  4. Chong EJ, Phan TT, Lim IJ, Zhang YZ, Bay BH, Ramakrishna S, et al. Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomater 2007; 3(3): 321-30.
  5. Gautam S, Dinda AK, Mishra NC. Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method. Mater Sci Eng C Mater Biol Appl 2013; 33(3): 1228-35.
  6. Venkatesan J, Kim SK. Nano-hydroxyapatite composite biomaterials for bone tissue engineering--a review. J Biomed Nanotechnol 2014; 10(10): 3124-40.
  7. Chen JP, Chang YS. Preparation and characterization of composite nanofibers of polycaprolactone and nanohydroxyapatite for osteogenic differentiation of mesenchymal stem cells. Colloids Surf B Biointerfaces 2011; 86(1): 169-75.
  8. Causa F, Netti PA, Ambrosio L, Ciapetti G, Baldini N, Pagani S, et al. Poly-epsilon-caprolactone/ hydroxyapatite composites for bone regeneration: in vitro characterization and human osteoblast response. J Biomed Mater Res A 2006; 76(1): 151-62.
  9. Lien YH, Wu JH, Liao JW, Wu TM. In vitro evaluation of the thermosensitive and magnetic nanoparticles for the controlled drug delivery of vitamin D3. Macromol Res 2013; 21(5): 511-8.
  10. Curtis KM, Aenlle KK, Roos BA, Howard GA. 24R,25-dihydroxyvitamin D3 promotes the osteoblastic differentiation of human mesenchymal stem cells. Mol Endocrinol 2014; 28(5): 644-58.
  11. Liu H, Ding X, Zhou G, Li P, Wei X, Fan Y. Electrospinning of nanofibers for tissue engineering applications. J Nanomate 2013; 2013: 495708.
  12. Neppalli R, Marega C, Marigo A, Bajgai MP, Kim HY, Causin V. Improvement of tensile properties and tuning of the biodegradation behavior of polycaprolactone by addition of electrospun fibers. Polymer 2011; 52(18): 4054-60.
  13. Tetteh G, Khan AS, Delaine-Smith RM, Reilly GC, Rehman IU. Electrospun polyurethane/hydroxyapatite bioactive Scaffolds for bone tissue engineering: The role of solvent and hydroxyapatite particles. J Mech Behav Biomed Mater 2014; 39: 95-110.
  14. Jaiswal AK, Chhabra H, Soni VP, Bellare JR. Enhanced mechanical strength and biocompatibility of electrospun polycaprolactone-gelatin scaffold with surface deposited nano-hydroxyapatite. Mater Sci Eng C Mater Biol Appl 2013; 33(4): 2376-85.
  15. Hong S, Kim G. Fabrication of electrospun polycaprolactone biocomposites reinforced with chitosan for the proliferation of mesenchymal stem cells. Carbohydr Polym 2011; 83(2): 940-6.
  16. Geng S, Zhou S, Bi Z, Glowacki J. Vitamin D metabolism in human bone marrow stromal (mesenchymal stem) cells. Metabolism 2013; 62(6): 768-77.
Journal of Isfahan Medical School
Volume 35, Issue 425 - Serial Number 425
March and April 2017
Pages 387-392

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History

  • Receive Date: 07 January 2017
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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