Genetic Thromboembolic Risk Factors Associated with Deep Vein Throm-bosis in a Iranian Population

Document Type : Original Article(s)

Authors

1 Instructor, Department of Microbiology, Fasa University of Medical Sciences, (Member of Young Researchers Club, Islamic Azad University), Shiraz, Iran.

2 Student, Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.

3 Student of PhD, Research Center of Molecular Biology, School of Medicine, Baqiyatallah University, Tehran, Iran.

4 Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.

5 Pediatric Infectious Diseases Research Center, Shahid Beheshti Medical University, Tehran, Iran.

Abstract

Background: Salmonellosis is one of the most important prevalent bacterial infections, after a decade of its recognition. Antibiotic therapy in salmonellosis is restricted to typhoid fever and acute infections. After the first reporting of resistance in S. Typhimurium DT 104, nowadays, developing resistance in Salmonella, specially, ESBL (such as CTX-M) species is an important issue in salmonellosis. In this study, we evaluated bla-ctx-m-type gene in clinical isolates of Salmonella enterica.Methods: In this cross-sectional study, we collected clinical isolates of patients in 18 months. Initially, we surveyed drug sensitivity with disk diffusion method, and then determined MIC of resistant isolates with E-test stripes. The existence of ESBL (extended spectrum beta-lactamase) enzymes was examined by ESBL disks in Double Disk method, and these resistances was evaluated with PCR by using a pair of universal primers.Finding: 36 isolates were sensitive to all of antibiotics, but, in 60 isolates there was at least one resistance. In 45 isolates, there were multi drug resistance (MDR) phenotypes. Resistance to ampicilin was the highest percent, whereas, there was not any resistance to imipenem and ciprofloxacin, which were improved with E-test strips. ESBL evaluation, showed producing cefotaxime ESBL in 5 isolates and ceftazidim ESBL in 4 isolates; 2 isolates of these had bla-ctx-m-type gene in plasmid.Conclusion: Detection of MDR trait, especially extended spectrum beta-lactamases in resistant clinical Salmonella isolates, point to the attention in usage of extended spectrum cephalosporins and detection of resistance rate in a nation level.

Keywords

References

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Journal of Isfahan Medical School
Volume 28, Issue 116 - Serial Number 116
September and October 2011
Pages 987-996

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History

  • Receive Date: 29 January 2011
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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