The Effect of Aqueous Extract of Tamarix Dioica Roots on Liver Injury Induced by a High-Fat and High-Fructose Diet in Rats

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Basic Science, School of Veterinary Medicine, University of Zabol, Zabol, Iran

2 Assistant Professor, Department of Pathobiology, School of Veterinary, University of Zabol, Zabol, Iran

Abstract

Background: The herb Gaz, with the scientific name of Tamarix dioica, has many therapeutic applications in the traditional medicine of the Baluchistan region in Iran. The present research aimed to investigate the effects of the aqueous extract of Tamarix dioica in rats fed a high-fat and high-fructose diet.Methods: 30 male rats were divided into three groups. The control group received a normal diet throughout the period of study. The second group received a high-fat and high-fructose diet (HFHF) for two months. The third group received a HFHF diet for two months, and were treated orally by the aqueous extract of Tamarix dioica roots (200 mg/kg body weight) from the 30th day. At the end of the experiment, blood samples were taken from the heart of rats to determine the serum biochemical parameters. After euthanasia, liver specimens were deposited in formalin and after staining with hematoxilin-eosine, were examined to determine histopathological lesions.Findings: Serum glucose levels were significantly higher in rats received a HFHF diet compared to that in the control group (P < 0.001). Rats fed a HFHF diet had a significantly higher serum cholesterol and triglyceride levels (P < 0.010) and lower serum HDL levels compared to the control rats. The administration of the Tamarix dioica extract significantly reduced serum glucose levels compared to the negative control group (P < 0.050). Oral administration of Tamarix dioica roots extract significantly reduced serum aspartate aminotransferase (AST) and serum alanine aminotransferase (ALT) compared to those in the negative control group (P < 0.010). Furthermore, treatment with the extract of Tamarix dioica significantly reduced serum cholesterol and triglyceride levels compared to the negative control rats (P < 0.050). In the histopathological study of livers, cytoplasmic fat deposition was prominently lower in Tamarix dioica-treated rats compared to the negative-control ones.Conclusion: It seems that the administration of Tamarix dioica roots extract can reduce liver injury caused by HFHF diet in rats.

Keywords

References

  1. Shattat G. F. A review article on hyperlipidemia: Types, treatments and new drug targets. Biomed Pharmacol J 2014;7(2): 399-409.
  2. Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm J 2016; 24(5): 547-53.
  3. Walsh JM, Pignone M. Drug treatment of hyperlipidemia in women. JAMA 2004; 291(18): 2243-52.
  4. Rouhi-Boroujeni H, Rouhi-Boroujeni H, Gharipour M, Mohammadizadeh F, Ahmadi S, Rafieian-Kopaei M. Systematic review on safety and drug interaction of herbal therapy in hyperlipidemia: a guide for internist. Acta Biomed 2015; 86(2): 130-6.
  5. Rosenberg WM, Voelker M, Thiel R, Becka M, Burt A, Schuppan D, et al. Serum markers detect the presence of liver fibrosis: A cohort study. Gastroenterology 2004; 127(6): 1704-13.
  6. Shweta, Prasad RR. Liver function tests in acute hepatitis in children. Int J Res Med Sc 2016; 4(8): 3184-7.
  7. Qaiser M. The genus Tamarix (Tamaricaceae) in Pakistan. Iran J Bot 1983: 2(1): 21-68.
  8. Khan S, Khan GM, Mehsud SU, Khan A. Antifungal activities of tamarix dioica-an in vitro study. Gomal J Med Sci 2004; 2(2): 40-2.
  9. Khan S, Ullah F, Mahmood T. In vitro antimicrobial and cytotoxic activity of Tamarix dioica Roxb. leaves. Turk J Biol 2013; 37(3): 329-35.
  10. Chandramohan R, Pari L. Protective effect of umbelliferone on high-fructose diet-induced insulin resistance and oxidative stress in rats. Biomedicine and Aging Pathology 2014; 4(1): 23-8.
  11. Auberval N, Dal S, Bietiger W, Pinget M, Jeandidier N, Maillard-Pedracini E, et al. Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet. Diabetol Metab Syndr 2014; 6: 130.
  12. Riaz U, Sadiq N, Tahir M, Rajpoot NN, Ali A. Hepatoprotective Effect of Tamarixdioica roots on acetaminophen induced hepatotoxicity in male mice. J Islam Int Med Coll 2018; 13(4): 189-93.
  13. Martin-Portugues ID, Hurtado LH, De la Cruz Vigo JL, Matos-Azevedo AM, Perez VÁ, Sanchez-Margallo FM. Effects of diet-induced early-stage obesity on a low-testosterone Gottingen Minipig. In: Watson RR, editor. Nutrition in the prevention and treatment of abdominal obesity. 2nd ed. Cambridge, MA: Academic Press; 2019. p. 437-48.
  14. Horlick L, Havel L. The effect of feeding propylthiouracil and cholesterol on the blood cholesterol and arterial intima in the rat. J Lab Clin Med 1948; 33(8): 1029-36.
  15. Merfort I, Buddrus J, Nawwar MAM, Lambert J. A triterpene from the bark of Tamarix aphylla. Phytochemistry 1992; 31(11): 4031-2.
  16. Dong R, Yang Y, Shen Z, Zheng C, Jin Z, Huang Y, et al. Forkhead box A3 attenuated the progression of fibrosis in a rat model of biliary atresia. Cell Death Dis 2017; 8(3): e2719.
  17. Marchesini G, Marzocchi R, Agostini F, Bugianesi E. Nonalcoholic fatty liver disease and the metabolic syndrome. Curr Opin Lipidol 2005; 16(4): 421-7.
  18. Assy N, Kaita K, Mymin D, Levy C, Rosser B, Minuk G. Fatty infiltration of liver in hyperlipidemic patients. Dig Dis Sci 2000; 45(10): 1929-34.
  19. Hebi M, Eddouks M. Hypolipidemic activity of Tamarix articulata Vahl. in diabetic rats. J Integr Med 2017; 15(6): 476-82.
  20. Hebi M, Hajji L, Eddouks M. Effect of Flavonoid-rich Extract of Tamarix Articulata Vahl. on Glucose and Lipid Metabolism in Normal and Diabetic Rats. Cardiovasc Hematol Agents Med Chem 2018; 16(2): 94-105.
  21. Samejo MQ, Sumbul A, Shah S, Memon SB, Chundrigar S. Phytochemical screening of Tamarix dioica Roxb. ex Roch. Journal of Pharmacy Research 2013; 7(2): 181-3.
  22. Li Y, Yao J, Han C, Yang J, Chaudhry MT, Wang S, et al. Quercetin, Inflammation and Immunity. Nutrients 2016; 8(3): 167.
  23. Lee JH, Zhou HY, Cho SY, Kim YS, Lee YS, Jeong CS. Anti-inflammatory mechanisms of apigenin: inhibition of cyclooxygenase-2 expression, adhesion of monocytes to human umbilical vein endothelial cells, and expression of cellular adhesion molecules. Arch Pharm Res 2007; 30(10): 1318-27.
  24. Abouzid S, Sleem A. Hepatoprotective and antioxidant activities of Tamarix nilotica flowers. Pharm Biol 2011; 49(4): 392-5.
  25. Urfi MK, Mujahid M, Rahman MA, Rahman MA. The Role of Tamarix gallica Leaves Extract in Liver Injury Induced by Rifampicin Plus Isoniazid in Sprague Dawley Rats. J Diet Suppl 2018; 15(1): 24-33.
Journal of Isfahan Medical School
Volume 37, Issue 537 - Serial Number 537
July and August 2019
Pages 890-896

Files

History

  • Receive Date: 14 June 2019
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics