Molecular Characterization of Clinical Leishmania Major Isolates Harboring ITS1 Homology Similar to the One in Crithidia spp.

Document Type : مقاله کوتاه

Authors

1 MSc Student, Department of Parasitology and Mycology, School of Medicine AND Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Associate Professor, Department of Parasitology and Mycology, School of Medicine AND Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

3 Associate Professor, Department of Biotechnology, School of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran

4 Associate Professor, Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

5 Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

6 Professor, Laboratory of Technologies of Information and Communication and Electrical Engineering (LaTICE), University of Tunis, Tunis, Tunisia

Abstract

Background: Leishmaniasis is caused by the protozoan Leishmania spp. In some loci from Iran, some isolates have ITS1 like the one in Crithidia spp. Therefore, in this study, we analyzed molecular characters of the mentioned isolates.Methods: DNA was extracted from all clinical isolates harboring ITS1 like the one in Crithidia spp. Amplification was performed using the specific primer pair of 13A/13B and followed by sequencing. The sequences were assessed using BLASTn, multiple alignment, T-coffee, and Vector NTI. Phylogenic tree was used for grouping and analyzing the isolates.Findings: Sequencing analyzing showed low heterogeneity among the mentioned isolates, but high heterogeneity between the mentioned isolates and the ones in genbank, therefore the mentioned isolates were grouped separately. Moreover, although ITS1 from the mentioned isolates were homolog to the ones in Crithodia spp., but 13A/13B loci on mitochondria showed homology with Leishmania.Conclusion: As Crithidia is a monoxenous, and its natural host is flies, finding its genes in the agents of cutaneous leishmaniasis may show these parasites may meet each other in sandfly. Till now, no sexual reproduction has been reported in Leishmania spp., therefore it is likely recombinant.

Keywords

References

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Journal of Isfahan Medical School
Volume 36, Issue 500 - Serial Number 500
September and October 2017
Pages 1261-1266

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History

  • Receive Date: 16 June 2018
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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