Detection of Macrolid, Lincosamide and Streptogramin Resistance Genes in Methicillin-Resistant Staphylococcus Epidermidis Strains Isolated from Clinical Samples in Isfahan City, Iran

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Microbiology, School of Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

2 Assistant Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Increased antibiotic resistance among nosocomial infections has made the treatment of these infections difficult. Staphylococcus epidermidis is also known as one of the most common causes of nosocomial infections. The aim of this study was to determine the prevalence of resistance to macrolide, lincosamid and streptogramin antibiotics, in methicillin-resistant Staphylococcus epidermidis (MRSE) strains isolated from clinical samples and to detect their related resistance genes.Methods: For a period of 8 months, from 250 clinical Staphylococcus strains, 100 isolates of Staphylococcus epidermidis were identified and the antibiotic susceptibility patterns of the isolates were determined using the disc diffusion method. MRSE samples were isolated by using the disk diffusion method for cefoxitin. The minimum inhibitory concentration (MIC) of erythromycin was determined using the micro dilution method. The frequency of inducible clindamycin resistance phenotype was detected via D test method and the resistance genes to MLSB were detected using polymerase chain reaction (PCR) method.Findings: Among 100 Staphylococcus epidermidis strains, 52 isolates were MRSE. The frequency of resistance phenotype iMLSB, MS and cMLSB were 17.3%, 13.4% and 48%, respectively. The frequency of the resistance genes ermC, msrA and ermA were 73%, 11.5% and 5.7%, respectively.Conclusion: The high frequency of the ermC gene among isolates is a serious warning to health systems; thus, use of convenient and effective treatment methods after antibiotic susceptibility tests is necessary.

Keywords

References

  1. Castro-Alarcon N, Ribas-Aparicio RM, Silva-Sanchez J, Calderon-Navarro A, Sanchez-Perez A, Parra-Rojas I, et al. Molecular typing and characterization of macrolide, lincosamide and streptogramin resistance in Staphylococcus epidermidis strains isolated in a Mexican hospital. J Med Microbiol 2011; 60(Pt 6): 730-6.
  2. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of Staphylococci: an evaluation of three different screening methods. Indian J Med Microbiol 2006; 24(1): 25-9.
  3. Fasih N, Irfan S, Zafar A, Khan E, Hasan R. Inducible clindamycin resistance due to expression of erm genes in Staphylococcus aureus: report from a tertiary care Hospital Karachi, Pakistan. J Pak Med Assoc 2010; 60(9): 750-3.
  4. Shantala GB, Adithi SS, Rahul Rao K, Nagarathnamma T. Detection of inducible Clindamycin resistance in clinical isolates of Staphylococcus aureus by the Disc Diffusion Induction Test. J Clin Diagn Res 2011; 5(1): 35-7.
  5. Aktas Z, Aridogan A, Kayacan CB, Aydin D. Resistance to macrolide, lincosamide and streptogramin antibiotics in staphylococci isolated in Istanbul, Turkey. J Microbiol 2007; 45(4): 286-90.
  6. Juyal D, Shamanth AS, Pal S, Sharma MK, Prakash R, Sharma N. The prevalence of inducible clindamycin resistance among staphylococci in a tertiary care hospital - a study from the garhwal hills of uttarakhand, India. J Clin Diagn Res 2013; 7(1): 61-5.
  7. Ozbek E, Temiz H, Tekay F, Kalayci R, Akkoc H. Phenotypical examination of the macrolide-lincosamide-streptogramin B resistance in Staphylococcus isolates. Afr J Microbiol Res 2012; 6(13): 3292-6.
  8. Tekin A, Dal T, Deveci O, Tekin R, Atmaca S, Dayan S. Assessment of methicillin and clindamycin resistance patterns in Staphylococcus aureus isolated from a tertiary hospital in Turkey. Infez Med 2013; 21(2): 111-6.
  9. Fluit AC, Visser MR, Schmitz FJ. Molecular detection of antimicrobial resistance. Clin Microbiol Rev 2001; 14(4): 836-71, table.
  10. Lina G, Quaglia A, Reverdy ME, Leclercq R, Vandenesch F, Etienne J. Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among staphylococci. Antimicrob Agents Chemother 1999; 43(5): 1062-6.
  11. Roberts MC, Sutcliffe J, Courvalin P, Jensen LB, Rood J, Seppala H. Nomenclature for macrolide and macrolide-lincosamide-streptogramin B resistance determinants. Antimicrob Agents Chemother 1999; 43(12): 2823-30.
  12. Zmantar T, Chaieb K, Ben AF, Ben Kahla-Nakbi A, Ben HA, Mahdouani K, et al. Multiplex PCR detection of the antibiotic resistance genes in Staphylococcus aureus strains isolated from auricular infections. Folia Microbiol (Praha) 2008; 53(4): 357-62.
  13. Adaleti R, Nakipoglu Y, Ceran N, Tasdemir C, Kaya F, Tasdemir S. Prevalence of phenotypic resistance of Staphylococcus aureus isolates to macrolide, lincosamide, streptogramin B, ketolid and linezolid antibiotics in Turkey. Braz J Infect Dis 2010; 14(1): 11-4.
  14. Eksi F, Gayyurhan ED, Bayram A, Karsligil T. Determination of antimicrobial susceptibility patterns and inducible clindamycin resistance in Staphylococcus aureus strains recovered from southeastern Turkey. J Microbiol Immunol Infect 2011; 44(1): 57-62.
  15. Clinical and Laboratory Standards Institute (CLSI). M07-A8. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically-approved standard. 8th ed. Wayne, PA: CLSI; 2009.
  16. Chaieb K, Zmantar T, Chehab O, Bouchami O, Ben HA, Mahdouani K, et al. Antibiotic resistance genes detected by multiplex PCR assays in Staphylococcus epidermidis strains isolated from dialysis fluid and needles in a dialysis service. Jpn J Infect Dis 2007; 60(4): 183-7.
  17. Sharma V, Jindal N, Devi P. Prevalence of methicillin resistant coagulase negative staphylococci in a tertiary care hospital. Iran J Microbiol 2010; 2(4): 185-8.
  18. Shoja S, Nahaei MR, Nahaei M. Detection of Inducible clindamycin resistance in Staphylococcus aureus and Staphylococcus epidermidis by using D-test. Pharmaceutical Sciences 2009; 15(1): 1-8.
  19. Nafisi M, Shariati L, Validi M, Karimi A. Prevalence of constitutive and inducible resistance to clindamycin in staphylococci isolates from Hajar and Kashani hospitals in Shahrekord 2008. J Shahrekord Univ Med Sci 2010; 12 (1): 13-20. [In Persian].
  20. El-Mahdy TS, Abdalla S, El-Domany R, Snelling AM. Investigation of MLS B and tetracycline resistance in coagulase-negative staphylococci isolated from the skin of Egyptian acne patients and controls. J Am Sci 2010; 6(11): 880-8.
  21. Cetin ES, Gunes H, Kaya S, Aridogan BC, Demirci M. Distribution of genes encoding resistance to macrolides, lincosamides and streptogramins among clinical staphylococcal isolates in a Turkish university hospital. J Microbiol Immunol Infect 2010; 43(6): 524-9.
  22. Abdollahi S, Ramazanzadeh R, Delami Khiabani Z, Kalantar E, Menbari S. Molecular Detection of inducible clindamycin resistance among staphylococcal strains isolated from hospital patient. J Ardabil Univ Med Sci 2013; 13(1): 59-68. [In Persian].
Journal of Isfahan Medical School
Volume 34, Issue 411 - Serial Number 411
November and December 2017
Pages 1507-1514

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History

  • Receive Date: 30 July 2016
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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