Document Type : Original Article (s)
Authors
1
PhD Student, Department of Medical Nanotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2
Professor, Department Of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background: Paraquat is a kind of herbicide which can cause cell damage by producing active radicals. On the other hand, the antioxidant activity of captopril due to its thiol content prevents cell damage caused by active radicals. The aim of this study was to evaluate the inhibitory effect of captopril on paraquat toxicity in G292 cell line. Methods: This study included 4 groups of control, different concentrations of paraquat (2.5, 5, and 10 mM), different concentrations of captopril (0.03, 0.06, 0.12, 0.25, and 0.5 mM), and combination of paraquat and captopril with different concentrations. The cells were placed in the well of 96-well tissue culture microplates with the density of 50,000 cells in each well. After 24 hours of incubation, toxicity tests were performed. Findings: Captopril increased the viability of cells in methylthiazol tetrazolium (MTT) assay but it did not have significant effects on nitric oxide production. It could also significantly reduce the cytotoxicity of paraquat. Conclusion: Although the antioxidant activity of captopril reduced the cytotoxicity of paraquat in G292 cell line, this reduction was not found to be related with nitric oxide production. Keywords: Paraquat, Captopril, Antioxidant
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