Cell Viability and Neural Markers Expression of Human Adipose-Derived Stem Cells after Neural Induction in Alginate Tridimensional Cell Culture

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Anatomical Sciencesand Molecular Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

2 Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 PhD Student, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Different studies have been done to obtain sufficient number of neuronal cells for treatment of central nervous system injuries. Adipose-derived stem cells have higher proliferation rate and more resistant to apoptosis. These cells can be induced to differentiate along several multilineage cells such as neuron-like cells. Alginate hydrogel is a polysaccharide polymer that has proper properties including biocompatibility with no immunogenicity. In this study, the effect of alginate hydrogel on the cell viability, proliferation rate and neurogenic differentiation of human adipose derived stem cells was evaluated at 14 days after induction.Methods: Adipose-derived stem cells isolated from human, were cultured in neural induction media and seeded into alginate hydrogel. The cell viability and differentiation efficiency of encapsulated cells were evaluated via doubling time and real time quantitative reverse transcription polymerase chain reaction (real time RT-PCR) analysis, respectively. The collected data was analyzed using one-way ANOVA test.Findings: The rate of proliferation of encapsulated cells significantly decreased as compared with control group (P < 0.001). The expression of nestin, glial fibrillary acidic protein (GFAP) and microtubule-associated protein 2 (MAP2) markers significantly decreased as compared with control group (P < 0.001).Conclusion: Altogether cell viability and proliferation rate of encapsulated cells in alginate hydrogel significantly decreased as compared with control group, but alginate hydrogel can promote neural differentiation of adipose-derived stem cells.

Keywords

References

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Journal of Isfahan Medical School
Volume 33, Issue 350 - Serial Number 350
July and August 2015
Pages 1491-1505

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  • Receive Date: 06 August 2014
  • Revise Date:
  • Accept Date: 06 April 2022

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