The Effect of 8 Weeks of High-Intensity Interval Training (HIIT) with Curcumin Supplementation on Expression of Mitochondrial Cardiomyocyte drp1 and MFN2 Genes in Male Stroke Model Rats

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Assistant Professor, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran

3 Professor, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran

Abstract

Background: This study aimed to assess the effect of 8 weeks of high-intensity interval training (HIIT) with curcumin supplementation on expression OF mitochondrial cardiomyocyte drp1 and MFN2 genes in male stroke model rats.Methods: In this experimental study, 32 male rats were randomly divided into 4 groups of HIIT, supplement, control and supplement + training. The exercise group was exposed to HIIT for 8 weeks. Training program in the two groups of HIIT and training + supplementation included performing 10 periods of 4-minute activities with an intensity of 85%-90% VO2max, and at this time, the supplementary group did not have training program. The expression of mitochondrial drp1 and MFN2 genes was obtained by real-time polymerase chain reaction (RT-PCR). Data were analyzed using one-way ANOVA and Shapiro-Wilk tests.Findings: The results of one-way ANOVA showed a significant difference between the means of all of the groups in expression of mitochondrial Drp1 and MFN2 genes. The results of Tukey test showed a significant decrease in Drp1 gene expression in both training and exercise + supplement groups compared to the supplement and control groups and a significant increase in MFN2 gene expression in both training and supplement + supplement groups compared to the supplement and control groups.Conclusion: HIIT with curcumin supplementation can improve the function of the cardiomyocytes' mitochondria of male myocardial infarction rats by modulating fusion and fission processes.

Keywords

References

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Journal of Isfahan Medical School
Volume 39, Issue 652 - Serial Number 652
November and December 2022
Pages 914-921

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History

  • Receive Date: 18 October 2021
  • Revise Date: 28 June 2022
  • Accept Date: 06 April 2022

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