Antibiofilm and Antibacterial Activity of Pistacia Atlantica against some Pathogenic Bacteria

Document Type : Original Article (s)

Authors

1 Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

2 Assistant Professor, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

3 Assistant Professor, Agricultural and Natural Resources Research Center of Lorestan, Khorramabad, Iran

Abstract

Background: Bacterial infections and the antibiotic resistance of pathogens are the most important challenges faced by researchers and physicians. In this study, the antibacterial and antibiofilm properties of methanol extracts of leaf, fruit, and gall of Pistacia atlantica were evaluated against some of pathogenic bacteria.Methods: Leaf, fruit, and gall of Pistacia atlantica were collected from forests of Lorestan Province, Iran. Antibacterial properties were investigated against Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli through the well diffusion method. The microdilution method was used to determine the minimum inhibitory concentration and the minimum bactericidal concentration. The antibiofilm activity of methanol extract was studied in the sub-lethal concentrations on biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa using microtiter plate method.Findings: The methanol extracts of leaf, fruit, and gall of Pistacia atlantica had significant antibacterial effects against the tested bacteria except Escherichia coli. There was a significant relationship between the antibacterial activity and the extract concentration (P < 0.05). The highest antibacterial effect was observed in the gall methanol extract. The minimum inhibitory concentration and the minimum bactericidal concentration of the methanol extract of leaf, fruit, and gall was 3.0-12.5 and 6.25-25.00 mg/ml, respectively. The methanol extract of Pistacia atlantica gall at a concentration of 6 mg/ml reduced the biofilm production of Staphylococcus aureus and Pseudomonas aeruginosa by 100% and 74%, respectively.Conclusion: The methanol extracts of different parts of Pistacia atlantica had antibacterial effects. In addition, the methanol extract reduced biofilm production of Staphylococcus aureus and Pseudomonas aeruginosa.

Keywords

References

  1. Shokri D, Rabbani-Khorasgani M. New molecular resistance mechanisms against antibiotics in bacteria. J Isfahan Med Sch 2015; 33(328): 410-28. [In Persian].
  2. Taj Y, Essa F, Aziz F, Kazmi SU. Study on biofilm-forming properties of clinical isolates of Staphylococcus aureus. J Infect Dev Ctries 2012; 6(5): 403-9.
  3. Wu H, Moser C, Wang HZ, Hoiby N, Song ZJ. Strategies for combating bacterial biofilm infections. Int J Oral Sci 2015; 7(1): 1-7.
  4. Solanki R. Some medicinal plants with antibacterial activity. Pharmacie Globale 2010; 1(4): 1-4.
  5. Aliasghari A, Rabbani M, Khoroushi M, Emami H. In-vitro effect of alcoholic extract of Rosa damascene extract on cariogenic streptococci. J Isfahan Med Sch 2015; 33(327): 326-35. [In Persian].
  6. Mahdavi A. The economic, social, and ecological impacts of wild pistachio (Pistacia atlantica Desf.) oleo-gum resin extraction cooperatives in Zagros forests, Ilam province, Iran. Forests, Trees and Livelihoods 2015; 24(4): 275-84.
  7. Taheri Abkenar K, Salehi A, Bagheri J, Ravanbakhsh H. Some ecological properties of Pistacia atlantica Desf. in Khojir National Park, Iran. Chinese Journal of Applied and Environmental Biology 2013; 19(3): 415-20.
  8. Martinez J-JI. Impact of a gall-inducing aphid on Pistacia atlantica Desf. trees. Arthropod Plant Interact 2008; 2(3): 147-51.
  9. Azmir J, Zaidul ISM, Rahman MM, Sharif KM, Mohamed A, Sahena F, et al. Techniques for extraction of bioactive compounds from plant materials: A review. J Food Eng 2013; 117(4): 426-36.
  10. Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 2008; 3(2): 163-75.
  11. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016; 6(2): 71-9.
  12. Pompilio A, Pomponio S, Di V, V, Crocetta V, Nicoletti M, Piovano M, et al. Antimicrobial and antibiofilm activity of secondary metabolites of lichens against methicillin-resistant Staphylococcus aureus strains from cystic fibrosis patients. Future Microbiol 2013; 8(2): 281-92.
  13. Pitts B, Hamilton MA, Zelver N, Stewart PS. A microtiter-plate screening method for biofilm disinfection and removal. J Microbiol Methods 2003; 54(2): 269-76.
  14. Karakas FP, Yildirim A, Turker A. Biological screening of various medicinal plant extracts for antibacterial and antitumor activities. Turk J Biol 2012; 36(6): 641-52.
  15. Edrah S, Alafid F, Kumar A. Preliminary Phytochemical Screening and Antibacterial Activity of Pistacia atlantica and Prunus persica Plants of Libyan Origin. International Journal of Science and Research 2013; 4(2): 1552-5.
  16. Mortazavi SH, Azadmard Damirchi S, Sowti M, Mahmudi R, Safaeean F, Moradi Azad S. Antimicrobial effects of ethanolic extract of the hull and the core of Pistacia Khinjuk stocks. Innovative Food Technologies 2014; 1(4): 81-8. [In Persian].
  17. Mudzengi CP, Murwira A, Tivapasi M, Murungweni C, Burumu JV, Halimani T. Antibacterial activity of aqueous and methanol extracts of selected species used in livestock health management. Pharm Biol 2017; 55(1): 1054-60.
  18. Roozegar MA, Azizi JF, Reza HM, Panahi J, Pakzad I. Antimicrobial effect of Pistacia atlantica leaf extract. Bioinformation 2016; 12(1): 19-21.
  19. Chaharmiri Dokhaharani S, Karbasizadeh V, Mohammadi-Sichani M, Tavakoli M. Antibacterial activity of aqueous extracts of Mazuj and Ghalghaf galls of Quercus infectoria in Lorestan forests . Yafte 2013; 15(2): 43-51. [In Persian].
  20. Omidi A, Sharifi A. The effect of methanolic extracts of plants quercus brantii, pistacia atlantica and elaeagnus angustifolia on biofilm formation of Pseudomonas aeruginosa. Armaghane-danesh 2017; 21(10): 999-1012. [In Persian].
  21. Mohammadi-Sichani M, Karbasizadeh V, Dokhaharani SC. Evaluation of biofilm removal activity of Quercus infectoria galls against Streptococcus mutans. Dent Res J (Isfahan) 2016; 13(1): 46-51.
Journal of Isfahan Medical School
Volume 36, Issue 482 - Serial Number 482
May and June 2018
Pages 594-600

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History

  • Receive Date: 27 January 2018
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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