Evaluation of Relative Frequency of Single Nucleotide Polymorphism G870A (rs9344) in CyclinD1 Gene in Colorectal Cancer Patients and Healthy Subjects of Isfahan Province, Iran

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Genetic, School of Sciences, Shahrekord University, Shahrekord, Iran

2 Professor, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Assistant Professor, Department of Genetics, School of Sciences, Shahrekord University, Shahrekord, Iran

Abstract

Background: Colorectal cancer (CRC) is one of the most common cancers in the world. High activity of CyclinD1 gene has been seen in the progress of this cancer. A common polymorphism (G870A) in exon 4 of CyclinD1 produces a variant transcript with longer half-life and may cause uncontrollable cellular growth thus contributing to cancer development. This study was performed to evaluate the frequency of CCND1 G870A polymorphism between CRC cases and controls.Methods: DNA samples from peripheral blood leukocytes of 50 patients with sporadic colorectal cancer and 50 healthy subjects were extracted. CCND1 G870A polymorphism was genotyped by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) in healthy subjects and patients. Statistical analysis was performed by chi-squared test via SPSS software.Findings: Relationship between CCND1 G870A polymorphism in allele frequencies between cases and controls were not observed (P = 0.204). While the frequency of AA genotype was significantly higher in patients (P = 0.040, 95% CI = 1.13-5.54, OR = 2.25).Conclusion: According to the significant association observed between certain genotypes of this locus (AA) with colorectal cancer, it can be used in the future as a good prognostic marker in CRC screening programs.

Keywords

References

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Journal of Isfahan Medical School
Volume 34, Issue 385 - Serial Number 385
May and June 2016
Pages 649-656

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History

  • Receive Date: 15 December 2015
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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