Evaluation of Specific Antibody against Recombinant CFP-10 Protein of Mycobacterium Tuberculosis in Patients with Tuberculosis

Document Type : Original Article (s)

Authors

1 Department of Microbiology, School of Advanced Sciences and Technology , Pharmaceutical Sciences Branch, Islamic Azad University ,Tehran, Iran

2 Assistant Professor, Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran

Abstract

Background: Tuberculosis (TB) has been remained as a major health problem. The most commonly used diagnostic tool for TB is a simple skin test. However, it has some disadvantages and it`s specificity can be compromised. Hence, detection of Mycobacterium tuberculosis specific antibodies in human sera has been considered as an important diagnostic test.Methods: In this study, humoral immune responses against recombinant CFP-10 protein of Mycobacterium tuberculosis in 51 patients with TB and 39 healthy subjects were evaluated using indirect enzyme-linked immunosorbent assay (ELISA).Findings: From 51 positive serum samples, 47 were positive and from 39 negative serum samples, 33 showed negative results. In this study, sensitivity and specificity of test were 92.15, 84.61 percent for dilution of 1:10 and 64.70 and 97.43 percent for dilution of 1:100, respectively. Accordingly, the estimated cut-off point was 0.351 and .025 for dilutions of 1:10 and 1:100, respectively.Conclusion: Our data suggested that the levels of antibodies against CFP-10 antigens in patients with TB were significantly higher than those in healthy subjects. This study demonstrated that ELISA with the use of the CFP-10 recombinant antigens is simple and sensitive and can be used to analyze large numbers of samples for the serodiagnosis of TB. 

Keywords

References

  1. Crowley L. An introduction to human disease: pathology and pathophysiology correlations. 8th ed. Sudbury, MA: Jones and Bartlett Learning; 2010.
  2. Mustafa AS. Mycobacterial gene cloning and expression, comparative genomics, bioinformatics and proteomics in relation to the development of new vaccines and diagnostic reagents. Med Princ Pract 2005; 14(Suppl 1): 27-34.
  3. Brodin P, Rosenkrands I, Andersen P, Cole ST, Brosch R. ESAT-6 proteins: protective antigens and virulence factors? Trends Microbiol 2004; 12(11): 500-8.
  4. Hill PC, Jackson-Sillah D, Fox A, Franken KL, Lugos MD, Jeffries DJ, et al. ESAT-6/CFP-10 fusion protein and peptides for optimal diagnosis of mycobacterium tuberculosis infection by ex vivo enzyme-linked immunospot assay in the Gambia. J Clin Microbiol 2005; 43(5): 2070-4.
  5. Porsa E, Cheng L, Seale MM, Delclos GL, Ma X, Reich R, et al. Comparison of a new ESAT-6/CFP-10 peptide-based gamma interferon assay and a tuberculin skin test for tuberculosis screening in a moderate-risk population. Clin Vaccine Immunol 2006; 13(1): 53-8.
  6. Kaufmann SHE, Rubin E. Handbook of tuberculosis: molecular biology and biochemistry. Weinheim, Germany: Wiley VCH; 2008.
  7. Ravn P, Munk ME, Andersen AB, Lundgren B, Lundgren JD, Nielsen LN, et al. Prospective evaluation of a whole-blood test using Mycobacterium tuberculosis-specific antigens ESAT-6 and CFP-10 for diagnosis of active tuberculosis. Clin Diagn Lab Immunol 2005; 12(4): 491-6.
  8. Harboe M. Antigens of PPD, old tuberculin, and autoclaved Mycobacterium bovis BCG studied by crossed immunoelectrophoresis. Am Rev Respir Dis 1981; 124(1): 80-7.
  9. Huebner RE, Schein MF, Bass JB, Jr. The tuberculin skin test. Clin Infect Dis 1993; 17(6): 968-75.
  10. van Pinxteren LA, Ravn P, Agger EM, Pollock J, Andersen P. Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10. Clin Diagn Lab Immunol 2000; 7(2): 155-60.
  11. Mazurek GH, Jereb J, Lobue P, Iademarco MF, Metchock B, Vernon A. Guidelines for using the QuantiFERON-TB Gold test for detecting Mycobacterium tuberculosis infection, United States. MMWR Recomm Rep 2005; 54(RR-15): 49-55.
  12. Mori T, Sakatani M, Yamagishi F, Takashima T, Kawabe Y, Nagao K, et al. Specific detection of tuberculosis infection: an interferon-gamma-based assay using new antigens. Am J Respir Crit Care Med 2004; 170(1): 59-64.
  13. Arend SM, Andersen P, van Meijgaarden KE, Skjot RL, Subronto YW, van Dissel JT, et al. Detection of active tuberculosis infection by T cell responses to early-secreted antigenic target 6-kDa protein and culture filtrate protein 10. J Infect Dis 2000; 181(5): 1850-4.
Journal of Isfahan Medical School
Volume 32, Issue 315 - Serial Number 315
November and December 2015
Pages 2226-2233

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History

  • Receive Date: 03 January 2015
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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