Cytotoxic Effect of Pluronic F127-Poly (Methyl Vinyl Ether-Alt-Maleic Acid) Micelles Loaded with Doxorubicin on MCF-7 Cells

Document Type : Original Article (s)

Authors

1 Professor, Department of Pharmaceutics, School of Pharmacy AND Novel Drug Delivery Systems Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

2 Assistant Professor, Department of Pharmaceutical Chemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

3 Professor, Department of Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

4 Student of Pharmacy, Department of Pharmaceutics, School of Pharmacy AND Novel Drug Delivery Systems Research Center AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Doxorubicin is used for treatment of different types of cancer including breast cancer. However, there are various side effects such as cardiotoxicity. Using a suitable drug delivery system such as polymeric micelles containing doxorubicin may reduce these effects.Methods: Micelles were prepared by direct dissolution method. After optimization according to their particle size, zeta potential, polydispersity index, loading efficiency and release of doxorubicin, micelles were used for further tests of cytotoxicity on MCF-7 cell line by 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay.Findings: The optimum targeted micelles with particle size of 419 ± 38 nm, zeta potential of -13.3 ± 1.2 mv, polydispersity index of 0.45 ± 0.019, loading efficiency of 93.5 ± 2.7 percent and drug release efficiency of 29.03 ± 3.7 percent in different concentrations of 0.21, 0.26, 0.34, 47.0 and 0.78 μM showed higher cytotoxicity than blank micelles and free drug.Conclusion: Pluronic F127-poly (methyl vinyl ether-alt-maleic acid) micelles showed more cytotoxicity than free drug against MCF-7 cell line.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 241 - Serial Number 241
May and June 2013
Pages 876-884

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History

  • Receive Date: 24 April 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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