Determining eaeA, hly, and flich7 Virulence Genes in Escherichia Coli Diarrheal Stool Samples

Document Type : Original Article (s)

Authors

1 Department of Microbiology, School of Basic Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran

2 Lecturer, Department of Microbiology, School of Basic Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran

3 Assistant Professor, Department of Microbiology, School of Basic Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran

Abstract

Background: Most of the Escherichia coli strains can cause intestinal and extra-intestinal diseases. Enterohemorragic Escherichia coli (EHEC) O157:H7 diseases have increased in developed countries, and today is the third cause of diarrhea. The purpose of this study was to investigate the eaeA, hly, and flich7 virulence genes in Escherichia coli isolated from diarrheal stool samples.Methods: After collecting 55 samples of human diarrhea in Tehran city hospitals, Iran, chemical and microbial tests for Escherichia coli strains were done, the bacteria were isolated and DNA was extracted using multiplex polymerase chain reaction (multiplex PCR) method.Findings: Of 55 human samples, 34 (61.8%) were of the flich7 gene, 11 (20.0%) were of the hly gene and 4 (7.2%) were of the eaeA gene.Conclusion: The controversy of the results of this study and other studies may be due to age, sample type, geographical location, type of diet, methods of detection and isolation, individual health status and even seasons of the year.

Keywords

References

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Journal of Isfahan Medical School
Volume 33, Issue 341 - Serial Number 341
May and June 2015
Pages 1037-1043

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History

  • Receive Date: 25 February 2015
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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