Antifungal Effect of Echinophora Platyloba Essence against the Candida Species Isolated from Vulvovaginal Candidiasis, Compared with Fluconazole

Document Type : Original Article (s)

Authors

1 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Assistant Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Professor, Department of Pharmacognosy, School of Pharmacology, Isfahan University of Medical Sciences, Isfahan, Iran

5 PhD Student, Department of Biostatistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Candidiasis is one of the most important opportunistic human infections. These infections have been increased due to overuse of antifungal drugs, especially fluconazole. The species has shown a relative resistance to fluconazole which is the first choice of vulvovaginal candidiasis treatment. Herbals are traditional source for medicinal drugs. Study on Echinophora platyloba, a domestic Iranian medicinal plant, could be helpful to find the alternative therapeutic agent for the cure of resistant infections.Methods: Clinical strains isolated from vaginal candidiasis which was identified via the method of Auxanogram with RapIDTM yeast plus system.Well-diffusion assay was used to determine the susceptibility of both fluconazole and essence of Echinophora platyloba on Candida species. For obtaining minimal inhibitory concentration of 50% (MIC50) and 90% (MIC90) and minimal fungicidal concentration (MFC), different dilutions were examined and compared on different species using microdilution of fluconzole and essence. The results were evaluated according to NCCLS (National Committee for Clinical Laboratory Standards) method.Findings: From a total of 15 isolates, 10 (66%) were sensitive to fluconazole but 3 (14%) isolates, Candida gabrata, Candida krusei and Candida rogusa, showed resistance to this drug; meanwhile, they were dose-dependent to the essence of Echinophora platyloba. One isolate (6%) was sensitive to essence of Echinophora platyloba, whereas 7 isolates (47%) were dose-dependent and 7 (47%) were resistant to this essence. MIC50, MIC90, MFC for fluconazole and essence was observed in isolate No. 31 (Candida albicans) and maximum concentration of them was related to No. 68 (Candida krusei).Conclusion: Using the essence of Echinophora platyloba along with the fluconazole would help to treat the resistant forms of candidiasis.

Keywords

References

  1. Calderone RA, Fonzi WA. Virulence factors of Candida albicans. Trends Microbiol 2001; 9(7): 327-35.
  2. Dan M, Kaneti N, Levin D, Poch F, Samra Z. Vaginitis in a gynecologic practice in Israel: causes and risk factors. Isr Med Assoc J 2003; 5(9): 629-32.
  3. Sobel JD, Wiesenfeld HC, Martens M, Danna P, Hooton TM, Rompalo A, et al. Maintenance fluconazole therapy for recurrent vulvovaginal candidiasis. N Engl J Med 2004; 351(9): 876-83.
  4. Akbarzadeh M, Bonyadpoure B, Pacshir K, Mohagheghzadeh A. Causes and clinical symptoms of vaginal candidiasis in patients referring to selective clinics of Shiraz University of Medical Sciences (2009). J Arak Univ Med Sci 2010; 13(3): 12-20. [In Persian].
  5. Ahmad S, Khan Z, Mustafa AS, Khan ZU. Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification. J Clin Microbiol 2002; 40(7): 2483-9.
  6. Anaissie EJ, McGinnis MR, Pfaller MA. Clinical mycology. Churchill Livingstone: Elsevier Health Sciences; 2009.
  7. Van Vuuren S. Antimicrobial activity of South African medicinal plants. Journal of Ethnopharmacology 2008; 119(3): 462-72.
  8. Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod 2007; 70(3): 461-77.
  9. Akhani H. Notes on the flora of Iran: 3. Two new records and synopsis of the new data on Iranian Cruciferae since Flora Iranica. Candollea 2003; 58(2): 369-85.
  10. Avijgan M, Saadat M, Nilforoosh Zadeh M, Hafizi M. Anti-fungal effect of Echinophora Platyloba extract on some common Dermatophytes. J Med Plants 2006; 5(18): 10-6.
  11. Haghighi F, Roudbar Mohammadi S, Farhadi Z. The effect of catechin on fungal biofilm formation of standard susceptible and resistant strains of Candida albicans. Armaghan Danesh 2011; 16(4): 332-40. [In Persian].
  12. Madani M,Khosravi Ar,Shirani M.Comparison of the invitro effection of Allium jesdianum extracts on candida. Journal Of Biology Science 2009; 3(1): 63-71. [In Persian].
  13. Avijgan M, Hafizi M, Saadat M, Nilforoushzadeh MA. Antifungal effect of Echinophora Platyloba's extract against Candida albicans. Iran J Pharm Res 2010; 5(4): 285-9. [In Persian].
  14. Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol 1999; 86(6): 985-90.
  15. Duarte MC, Figueira GM, Sartoratto A, Rehder VL, Delarmelina C. Anti-Candida activity of Brazilian medicinal plants. J Ethnopharmacol 2005; 97(2): 305-11.
  16. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4): 564-82.
  17. Fong HH. Integration of herbal medicine into modern medical practices: issues and prospects. Integr Cancer Ther 2002; 1(3): 287-93.
  18. Schultes RE. The kingdom of plants. In: Thomson WAE, editor. Medicines from the Earth. New York, NY: McGraw-Hill Book Co; 1978; 208.
  19. Rechinger KH, Hedge I. Flora Iranica, No. 162. Graz, Austria: Akademische Druck-u Verlagsanstalt; 1987. p. 428.
  20. Nourozi M. Evaluation of photochemical and antimicrobial effect of Echinophora platyloba [PhD Thesis]. Tehran, Iran: Tehran University of Medical Sciences; 1989. p. 35-40.[In Persian].
  21. Muzaffariyan VA. Dictionary of Iranian plant names. Tehran, Iran: Farhange Muaser; 1996. [In Persian].
  22. Asghari GR, Sajjadi SE, Sadraei H, Yaghobi K. Essential oil constituents of Echinophora platyloba DC. Iran J Pharm Res 2003; 2(3): 185-6.
  23. Clinical Laboratory Standard Institute (CLSI). Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved standard M27-A3. Wayne, PA: CLSI; 2008.
  24. Galan-Ladero MA, Blanco MT, Sacristan B, Fernandez-Calderon MC, Perez-Giraldo C, Gomez-Garcia AC. Enzymatic activities of Candida tropicalis isolated from hospitalized patients. Med Mycol 2010; 48(1): 207-10.
  25. Sobel JD. Vulvovaginal candidosis. Lancet 2007; 369(9577): 1961-71.
  26. Amouri I, Abbes S, Sellami H, Makni F, Sellami A, Ayadi A. The Vulvovaginal candidiasis: review. Journal of Medical Mycology 2010; 20(2): 108-15.
  27. Heywood VH. Flowering plants of the world. London, UK: BT Batsford Ltd; 1993.
  28. Renault S, De Lucca AJ, Boue S, Bland JM, Vigo CB, Selitrennikoff CP. CAY-1, a novel antifungal compound from cayenne pepper. Med Mycol 2003; 41(1): 75-81.
  29. Stewart PS. Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol 2002; 292(2): 107-13.
  30. Chevallier A. Encyclopedia of herbal medicine. London, UK: Dorling Kindersley Publishing; 2000.
  31. Avijgan M, Saadat M and Nilforoushzadeh MA. Effect of extract of Echinophora Platyloba on some common dermatophyts. Proceeding of the 2nd Symposium of Medicinal Plants; 2005 Jan 26-27; Tehran, Iran.
  32. Arbabi Klati h, Shirzaee M, Poorzamani M, Dabiri S. Inhibitory effects of plant extracts containing thyme, clore and cinnamon compared to nystatin on Candida albicans. (Invitro). J Res Dent Sci 2012; 8(4): 175-9.
  33. Hadizadeh I, Peivastegan B, Hamzehzarghani H. Antifungal activity of essential oils from some medicinal plants of Iran against( Alternaria alternate). American Journal of Applied Sciences 2009; 6(5): 744-8.
Journal of Isfahan Medical School
Volume 32, Issue 304 - Serial Number 304
September and October 2014
Pages 1646-1658

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History

  • Receive Date: 04 July 2014
  • Revise Date: 02 November 2024
  • Accept Date: 06 April 2022

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