Protective Effects of Manganese on the Testis Structure and Sperm Parameters of Formalin-Treated Mice

Document Type : Original Article (s)

Authors

1 Department of Basic Sciences, Payam-e-Noor University, Tehran, Iran

2 Assistant Professor, Department of Basic Sciences, Payam-e-Noor University, Babol, Iran

3 Pediatric Neuro-Rehabilitation Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran

4 Assistant Professor, Department of Anatomical Science, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

5 Assistant Professor, Celular and Mulecaular Biology Research Center AND Department of Genetics and Molecular Biology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

6 Professor, Celular and Mulecaular Biology Research Center AND Department of Anatomical Science, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

7 Assistant Professor, Celular and Mulecaular Biology Research Center AND Department of Anatomical Science, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Abstract

Background: Formaldehyde, as a ubiquitous environmental pollutant, is extensively used in industrial and hospitals settings. The aim of this study was to investigate the protective role of manganese on testis structure and sperm parameters in adult mice exposed to formaldehyde (FA).Methods: After dose optimizing for FA and manganese chloride, twenty adult male NMRI mice were selected and randomly divided into four groups: (i) control, (ii) sham, (iii) FA-exposed, and (iv) FA and manganese chloride exposed. The FA-exposed groups received 10 mg/ kg FA daily for 14 days, and 5 mg/kg manganese chloride was just injected intraperitoneally on 2nd week. Mice were sacrificed, and spermatozoa were collected from the cauda of the right epididymis and analyzed for count, motility, morphology and viability. The other testicular tissues were weighed and prepared for histological examination upon removal. Seminiferous tubules, lumen diameters and epithelium thickness were also measured.Findings: The findings revealed that FA significantly reduced the testicular weight, sperm count, motility, viability and normal morphology compared with control group (P ≤ 0.05). In addition, seminiferous tubules atrophied and seminiferous epithelial cells disintegrated in the FA group in comparison with the control group (P ≤ 0.05). However, manganese improved the testicular structure and sperm parameters in FA-treated mice testes (P ≤ 0.05).Conclusion: According to the results, manganese has protective effects on mice epididymal sperm parameters and testis structure treated with FA.

Keywords

References

  1. Osinowo OA, Bale JO, Oyedipe EO, Eduvie LO. Motility and eosin uptake of formaldehyde-treated ram spermatozoa. J Reprod Fertil 1982; 65(2): 389-94.
  2. Tang M, Xie Y, Yi Y, Wang W. Effects of formaldehyde on germ cells of male mice. Wei Sheng Yan Jiu 2003; 32(6): 544-8. [In Chinese].
  3. Gules O, Eren U. The effect of xylene and formaldehyde inhalation on testicular tissue in rats. Asian-Aust J Anim Sci 2010; 23(11): 1412-20.
  4. Zhou DX, Qiu SD, Zhang J, Tian H, Wang HX. The protective effect of vitamin E against oxidative damage caused by formaldehyde in the testes of adult rats. Asian J Androl 2006; 8(5): 584-8.
  5. Ruder EH, Hartman TJ, Blumberg J, Goldman MB. Oxidative stress and antioxidants: exposure and impact on female fertility. Hum Reprod Update 2008; 14(4): 345-57.
  6. Kodama H, Yamaguchi R, Fukuda J, Kasai H, Tanaka T. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril 1997; 68(3): 519-24.
  7. Donnelly ET, McClure N, Lewis SE. Antioxidant supplementation in vitro does not improve human sperm motility. Fertil Steril 1999; 72(3): 484-95.
  8. Arabi M, Sanyal SN, Kanwar U, Anand RJK. Effect of antioxidants on nicotine and caffeine induced changes in human sperm: an in vitro study. In: De Vriese SR, Christophe AB, editors. Male fertility and lipid metabolism. Urbana, IL: The American Oil Chemists Society; 2003: 250-69.
  9. Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes. Adv Exp Med Biol 2008; 636: 154-71.
  10. Bilaspuri G, Bansal AK. Mn2+: a potent antioxidant and stimulator of sperm capacitation and acrosome reaction in crossbred cattle bulls. Archiv fur Tierzucht 2008; 51(2): 149.
  11. Bansal A, Bilaspuri G. Effect of manganese on bovine sperm motility, viability, and lipid peroxidation in vitro. Animal Reprod 2008; 5(3-4): 90-6.
  12. Lapointe S, Ahmad I, Buhr MM, Sirard MA. Modulation of postthaw motility, survival, calcium uptake, and fertility of bovine sperm by magnesium and manganese. J Dairy Sci 1996; 79(12): 2163-9.
  13. Sari DK, Kuwahara S, Tsukamoto Y, Hori H, Kunugita N, Arashidani K, et al. Effect of prolonged exposure to low concentrations of formaldehyde on the corticotropin releasing hormone neurons in the hypothalamus and adrenocorticotropic hormone cells in the pituitary gland in female mice. Brain Res 2004; 1013(1): 107-16.
  14. Gurel A, Coskun O, Armutcu F, Kanter M, Ozen OA. Vitamin E against oxidative damage caused by formaldehyde in frontal cortex and hippocampus: biochemical and histological studies. J Chem Neuroanat 2005; 29(3): 173-8.
  15. Yu LQ, Jiang SF, Leng SG, He FS, Zheng YX. Early genetic effects on workers occupationally exposed to formaldehyde. Zhonghua Yu Fang Yi Xue Za Zhi 2005; 39(6): 392-5. [In Chinese].
  16. Wong EY, Ray R, Gao DL, Wernli KJ, Li W, Fitzgibbons ED, et al. Reproductive history, occupational exposures, and thyroid cancer risk among women textile workers in Shanghai, China. Int Arch Occup Environ Health 2006; 79(3): 251-8.
  17. Russell LD, Ettlin RA, Sinha-Hikim AP, Clegg ED. Mammalian spermatogenesis. In: Russell LD, Ettlin RA, Sinha-Hikim AP, Clegg ED, editors. Histological and Histopathological Evaluation of the Testis. Clearwater, FL: Cache River Press; 1990. p. 1-40.
  18. Majumder PK, Kumar VL. Inhibitory effects of formaldehyde on the reproductive system of male rats. Indian J Physiol Pharmacol 1995; 39(1): 80-2.
  19. Saito Y, Nishio K, Yoshida Y, Niki E. Cytotoxic effect of formaldehyde with free radicals via increment of cellular reactive oxygen species. Toxicology 2005; 210(2-3): 235-45.
  20. Urata K, Narahara H, Tanaka Y, Egashira T, Takayama F, Miyakawa I. Effect of endotoxin-induced reactive oxygen species on sperm motility. Fertil Steril 2001; 76(1): 163-6.
  21. Aitken RJ. A free radical theory of male infertility. Reprod Fertil Dev 1994; 6(1): 19-23.
  22. Bilodeau JF, Blanchette S, Cormier N, Sirard MA. Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase. Theriogenology 2002; 57(3): 1105-22.
  23. Woo AL, James PF, Lingrel JB. Sperm motility is dependent on a unique isoform of the Na,K-ATPase. J Biol Chem 2000; 275(27): 20693-9.
  24. Verma A, Kanwar KC. Human sperm motility and lipid peroxidation in different ascorbic acid concentrations: an in vitro analysis. Andrologia 1998; 30(6): 325-9.
  25. Fujii J, Iuchi Y, Matsuki S, Ishii T. Cooperative function of antioxidant and redox systems against oxidative stress in male reproductive tissues. Asian J Androl 2003; 5(3): 231-42.
  26. Ozen OA, Akpolat N, Songur A, Kus I, Zararsiz I, Ozacmak VH, et al. Effect of formaldehyde inhalation on Hsp70 in seminiferous tubules of rat testes: an immunohistochemical study. Toxicol Ind Health 2005; 21(10): 249-54.
  27. Zhou DX, Qiu SD, Wang ZY, Zhang J. Effect of tail-suspension on the reproduction of adult male rats. Zhonghua Nan Ke Xue 2006; 12(4): 326-9. [In Chinese].
  28. D'Souza UJ. Tamoxifen induced multinucleated cells (symplasts) and distortion of seminiferous tubules in rat testis. Asian J Androl 2003; 5(3): 217-20.
  29. Chowdhury AR, Gautam AK, Patel KG, Trivedi HS. Steroidogenic inhibition in testicular tissue of formaldehyde exposed rats. Indian J Physiol Pharmacol 1992; 36(3): 162-8.
  30. Feldman MY. Reactions of nucleic acids and nucleoproteins with formaldehyde. Prog Nucleic Acid Res Mol Biol 1973; 13: 1-49.
  31. Tramer F, Rocco F, Micali F, Sandri G, Panfili E. Antioxidant systems in rat epididymal spermatozoa. Biol Reprod 1998; 59(4): 753-8.
  32. Chen MT, Cheng GW, Lin CC, Chen BH, Huang YL. Effects of acute manganese chloride exposure on lipid peroxidation and alteration of trace metals in rat brain. Biol Trace Elem Res 2006; 110(2): 163-78.
  33. Elbetieha A, Bataineh H, Darmani H, Al-Hamood MH. Effects of long-term exposure to manganese chloride on fertility of male and female mice. Toxicol Lett 2001; 119(3): 193-201.
  34. Eybl V, Kotyzova D. Protective effect of manganese in cadmium-induced hepatic oxidative damage, changes in cadmium distribution and trace elements level in mice. Interdiscip Toxicol 2010; 3(2): 68-72.
  35. Guraya SS. Cellular and molecular biology of capacitation and acrosome reaction in spermatozoa. Int Rev Cytol 2000; 199: 1-64.
  36. Lindemann CB, Goltz JS. Calcium regulation of flagellar curvature and swimming pattern in triton X-100--extracted rat sperm. Cell Motil Cytoskeleton 1988; 10(3): 420-31.
  37. Tarin JJ, Perez-Albala S, Cano A. Consequences on offspring of abnormal function in ageing gametes. Hum Reprod Update 2000; 6(6): 532-49.
  38. Jauniaux E, Hempstock J, Greenwold N, Burton GJ. Trophoblastic oxidative stress in relation to temporal and regional differences in maternal placental blood flow in normal and abnormal early pregnancies. Am J Pathol 2003; 162(1): 115-25.
  39. Sikka SC. Oxidative stress and role of antioxidants in normal and abnormal sperm function. Front Biosci 1996; 1: e78-e86.
  40. de LE, Gagnon C. Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod 1995; 10 Suppl 1: 15-21.
  41. Zhou DX, Qiu SD, Zhang J, Wang ZY. Reproductive toxicity of formaldehyde to adult male rats and the functional mechanism concerned. Sichuan Da Xue Xue Bao Yi Xue Ban 2006; 37(4): 566-9. [In Chinese].
Journal of Isfahan Medical School
Volume 31, Issue 243 - Serial Number 243
May and June 2013
Pages 1018-1032

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History

  • Receive Date: 01 March 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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