Evaluation of the Effect of Hydroalcoholic Extract of Dracocephalum Kotschyi Boiss on Hepatic Fibrosis Induced by Carbon Tetrachloride in Rats

Document Type : Original Article (s)

Authors

1 Pharmacy Student, Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

3 Assistant Professor, Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Liver disease eventually leads to fibrosis, which is a type of collagenized tissue. D.kotschyi has compounds that have strong anti-inflammatory and anti-fibrotic properties. Due to the inflammatory nature of fibrosis, this study aims to investigate the effect of different doses of hydroalcoholic extract of D.kotschyi on liver fibrosis induced by carbon tetrachloride.
Methods: The D.kotschyi hydroalcoholic extract was prepared via maceration method in 70% ethanol. The groups (6 adult male rats in each) of animals included: Sham, Control, Tetrachloride carbon (CCl4), vitamin E as well as extracts plus CCl4. The extract of D.kotschyi was gavaged at 20, 40 or 80 mg/kg. In addition, the diluted CCl4 in olive oil (1:1) was injected at 1ml/kg intraperitoneally. The duration of treatment was 1 month. Then the blood sample and liver tissue of rats were extracted for biochemical and pathological examination.
Findings: Administration of the D.kotschyi for 1 month significantly reduced the level of Alkaline phosphatase (ALP), Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) liver enzymes compared to CCl4 treated group (P < 0.001) as well as caused a significant improvement in the pathological features of fibrosis.
Conclusion: The present study showed a significant reduction in the amount of hepatic fibrosis caused by carbon tetrachloride in the pathology samples receiving the D.kotschyi extract. In addition, by increasing dose of the D.kotschyi, a significant decrease in liver enzymes was observed. The effect of the D.kotschyi in improving fibrosis at higher doses requires extensive studies.

Keywords

References

  1. Dadban-Shahamat M, Shakki M, Hashemi-Chashmi SZ. The effect of high-intensity intermittent exercise with silymarin supplementation on liver enzymes in male rats of fatty liver model [In Persian]. J Isfahan Med Sch 2021; 38(606): 989-95.
  2. Bataller R, Brenner DA. Liver fibrosis. J Clin Invest 2005; 115(2): 209-18.
  3. Lee YA, Wallace MC, Friedman SL. pathobiology of liver fibrosis: a translational success story. Gut 2015; 64(5): 830-41.
  4. Huizen J. What is liver fibrosis? [Online] 2019 [cited 2019 Apr 30]. Available from: URL: http://www.medicalnewstoday.com/articles/325073
  5. Francisco V, Figueirinha A, Neves BM, García-Rodríguez C, Lopes MC, Cruz MT, et al. cymbopogon citratus as source of new and safe anti-inflammatory drugs: bio-guided assay using lipopolysaccharide-stimulated macrophages.
    J Ethnopharmacol 2011; 133(2): 818-27.
  6. Adeneye AA, Agbaje EO. Hypoglycemic and hypolipidemic effects of fresh leaf aqueous extract of Cymbopogon citratus Stapf. in rats.
    J Ethnopharmacol 2007; 112(3): 440-4.
  7. Zeng Q, Jin HZ, Qin JJ, Fu JJ, Hu XJ, Liu JH, et al. Chemical constituents of plants from the genus Dracocephalum. Chem Biodivers 2010; 7(8): 1911-29.
  8. Negrelle R, Gomes EC. Cymbopogon citratus (DC.) stapf: chemical composition and biological activities. Revista Brasileira de Plantas Medicinais 2007; 9(1): 80-92.
  9. Sawyerr E. traditional medicine in Sierra Leone-a critical appraisal. Nigerian Journal of Pharmacy 1982; 13(5): 28-33.
  10. Lee TY, Wang G-J, Chiu JH, Lin HC. Long-term administration of salvia miltiorrhiza ameliorates carbon tetrachloride-induced hepatic fibrosis in rats.
    J Pharm Pharmacol 2003; 55(11) :1561-8.
  11. Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, et al. the role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci 2015; 16(11): 26087-124.
  12. Ningsih IY, Zulaikhah S, Hidayat MA, Kuswandi B.
    Antioxidant activity of various kenitu (Chrysophyllum cainito L.) leaves extracts from Jember, Indonesia. Agriculture and Agricultural Science Procedia 2016; 9(8): 378-85.
  13. Fattahi M, Nazeri V, Torras-Claveria L, Sefidkon F, Cusido RM, Zamani Z, et al. Identification and quantification of leaf surface flavonoids in wild-growing populations of Dracocephalum kotschyi by LC-DAD-ESI-MS. Food chemistry 2013; 141(1): 139-46.
  14. Hosseini-Sharifabad A, Sadraei H, Hashemnia M, Sajjadi SE, Mirdamadi Z. Effect of hydroalcoholic and aqueous extracts of Dracocephalum kotschyi on bleomycin induced pulmonary fibrosis. J Herbmed Pharmacol 2021; 10(2): 209-17.
  15. Guo Y, Liang X, Meng M, Chen H, Wei X, Li M. Hepatoprotective effects Yulangsan flavone against carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats. Phytomedicine 2017; 15(33): 28-35.
  16. Ishikawa M, Saito K, Yamada H, Nakatsu N, Maekawa K, Saito Y. Plasma lipid profiling of different types of hepatic fibrosis induced by carbon tetrachloride and lomustine in rats. Lipids Health Dis 2016; 15: 74.
  17. Ishak K, Baptista A, Bianchi L, Callea F, De Groote J, Gudat F, et al. Histological grading and staging of chronic hepatitis. J Hepatol 1995; 22(6): 696-9.
  18. Talari M, Seydi E, Salimi A, Mohsenifar Z, Kamalinejad M, Pourahmad J. Dracocephalum: novel anticancer plant acting on liver cancer cell mitochondria. Biomed Res Int 2014; 2014: 892170.
  19. Ayeleso TB, Matumba MG, Mukwevho E. Oleanolic acid and its derivatives: biological activities and therapeutic potential in chronic diseases. Molecules 2017; 22(11): 1915.
  20. Liu G, Zhang Y, Liu C, Xu D, Zhang R, Cheng Y,
    et al. luteolin alleviates alcoholic liver disease induced by chronic and binge ethanol feeding in mice. J Nutr 2014; 144(7): 1009-15.
  21. Sadraei H, Asghari G, Khanabadi M, Minaiyan M. Anti-inflammatory effect of apigenin and hydroalcoholic extract of Dracocephalum kotschyi on acetic acid-induced colitis in rats. Res Pharm Sci 2017; 12(4): 322-9.
Journal of Isfahan Medical School
Volume 40, Issue 672
4th Week, July
July and August 2022
Pages 356-362

Files

History

  • Receive Date: 25 July 2022
  • Accept Date: 25 July 2022

Share

How to cite

Statistics