Evaluation of Microorganism Colonization on Laparoscopic Instruments after Surgery and Following Two Different Cleaning Methods

Document Type : Original Article (s)

Authors

1 Department of Operating Room, School of Nursing and Midwifery, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Instructor, Department of Operating Room, School of Nursing and Midwifery, Isfahan University of Medical Sciences, Isfahan, Iran

4 Assistant Professor, Nursing and Midwifery Care Research Center AND Department of Operating Room, School of Nursing and Midwifery, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: An accurate cleaning, as the first step of reprocessing, would make the sterilization more effective, and protects healthcare providers and patients against infections. The present study aimed to compare the effect of two cleaning methods of conventional and the Association for the Advancement of Medical Instrumentation (AAMI) on the reduction of microorganism colonization on laparoscopic instruments.Methods: In this quasi-experimental study, 128 laparoscopic instruments was randomly divided into two groups of conventional and AAMI cleaning protocols. Sampling was done immediately after surgery and the cleaning process. The number and types of microorganisms which isolated from the Sabouraud Dextrose Agar, Blood Agar and MacConkey Agar media were determined.Findings: The total mean number of microorganisms isolated from instruments was 24 × 105 colony forming unit (CFU)/100 ml immediately after surgery. After cleaning process, it was reduced to 7.2 × 105 CFU/100 ml and 0.34 × 105 CFU/100 ml in conventional and AAMI groups, respectively. This reduction was significantly higher in the AAMI group than in the conventional cleaning group (P < 0.050). The most frequent type of the microorganisms isolated after surgery were as Escherichia coli (81.2%), Pseudomonas aeroginosa (68.8%), and Klebsiella spp. (57.8%), respectively.Conclusion: Reduction of total mean number and frequency of the microorganisms after the cleaning process were higher in the AAMI group than in conventional group. Therefore, the AAMI method may prevent hospital-acquired infection, and is recommended as an effective cleaning method for laparoscopic instruments after surgery.

Keywords

References

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Journal of Isfahan Medical School
Volume 36, Issue 499 - Serial Number 499
September and October 2018
Pages 1197-1205

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History

  • Receive Date: 01 August 2018
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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