The Effect of the Blockage of the Receptors of Glutamate N-Methyl-D-Aspartate (NMDA) in Prefrontal Cortex on Morphine Tendency in Male Rat

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Biology, School of Science, Fars Science and Research Branch, Islamic Azad University AND PhD Student, Department of Biology, School of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran

2 Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Department of Biology, School of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran

4 Assistant Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Addiction is a brain disorder associated with neurobiologic changes. Medial prefrontal cortex (mPFC) plays an important role in learning, memory, reward system and addiction processes. Present study investigated the effect of 2-amino-5-phosphopentanoic acid (AP5), glutamate receptor antagonist, on morphine tendency.Methods: We used 27 groups (n = 6) of male Wistar rats; 7 groups were used for determining the effective and ineffective doses of morphine and 20 experimental groups also were used for studying the effect of various doses of AP5 on morphine tendency in acquisition and expression phases of conditioned place preference (CPP) paradigm; then, CPP index was calculated.Findings: Doses of 2.5, 5, 7.5 and 10 mg/kg of morphine significantly increased CPP index in morphine groups compared to saline group (P < 0.050, P < 0.001, P < 0.010 and P < 0.001, respectively) but, in doses of 0.5 and 1 mg/kg of morphine, the changes were not significant. Microinjection of different doses of AP5 into the prefrontal cortex with injection of the effective dose of morphine significantly reduced the acquisition and the expression phases of CPP in test groups compared to the sham group, while the ineffective dose of morphine reduced the expression phase of CPP paradigm (P < 0.001) and did not have significant effect on the acquisition phase of CPP.Conclusion: It seems that microinjection of AP5 into the prefrontal cortex reduces CPP index and consequently morphine tendency; these may be due to the impairment of learning and dysfunction of reward-related memory in response to morphine.

Keywords

References

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Journal of Isfahan Medical School
Volume 34, Issue 400 - Serial Number 400
July and August 2016
Pages 1114-1120

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History

  • Receive Date: 11 June 2016
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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