Identifying Ubiquitin D in Paraffin-Embedded Tissues in Patients with Colorectal Cancer

Document Type : Original Article (s)

Authors

1 Young Researchers and Elite Club, Islamic Azad University, East Tehran Branch, Tehran, Iran

2 Assistant Professor, Department of Biology, School of Basic Sciences, Islamic Azad University, East Tehran Branch, Tehran, Iran

3 Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Associate Professor, Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5 Assistant Professor, Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan AND Banej Exir Research Center, Tehran, Iran

6 Department of Hematology, Immunology Laboratory, Sajad Hospital AND Banej Exir Research Center, Tehran, Iran

Abstract

Background: Colon cancer is one major cause for cancer-related deaths. Ubiquitin D (UBD) and its alike proteins are signal transduction pathways with several cellular functions including DNA repair control. There is relation between increase in ubiquitin D and increase in p53 expression in a way that in many cases, this growth has accelerated the cancer progress. This study aimed to detect ubiquitin D, as a marker for the early detection of colorectal cancer and disease progress in patients.Methods: 30 samples of paraffin-embedded tissue blocks of patients with colorectal cancer and 10 normal tissue samples were studied. Reverse transcription-Polymerase chain reaction (RT-PCR) testing to detect ubiquitin D gene and b-actin gene as an internal control was obtained.Findings: The size of RT-PCR product of ubiquitin D gene was 395 and the size of amplified b-actin was 350 bp. The RT-PCR of b-actin was positive in all samples. The RT-PCR products of 18 samples (95%) of male patients and 11 samples (100%) of female patients were positive. Only one sample had negative product.Conclusion: Considering the results, we can conclude that ubiquitin D, as a useful marker that is independent of tumor stage, plays an important role in the detection of colorectal cancer and its study is really important in identification and detection of early stages of disease. Results showed that the expression of the ubiquitin D in patients is 97% and it reduces to 5% in healthy people.

Keywords

References

  1. Huang WS, Tang R, Lin PY, Changchien CR, Chen JS, Chiang JM, et al. Impact of the cyclin D1 A870G polymorphism on susceptibility to sporadic colorectal cancer in Taiwan. Dis Colon Rectum 2006; 49(5): 602-8.
  2. Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ. Cancer statistics, 2009. CA Cancer J Clin 2009; 59(4): 225-49.
  3. Yaylim-Eraltan I, Arikan S, Yildiz Y, Cacina C, Ergen HA, Tuna G, et al. The influence of cyclin D1 A870G polymorphism on colorectal cancer risk and prognosis in a Turkish population. Anticancer Res 2010; 30(7): 2875-80.
  4. Zhang S, Cui Y, Weng Z, Gong X, Chen M, Zhong B. Changes on the disease pattern of primary colorectal cancers in Southern China: a retrospective study of 20 years. Int J Colorectal Dis 2009; 24(8): 943-9.
  5. Zhang LQ, Wang J, Shang JQ, Bai JL, Liu FY, Guan X, et al. Cyclin D1 G870A polymorphism and colorectal cancer susceptibility: a meta-analysis of 20 populations. Int J Colorectal Dis 2011; 26(10): 1249-55.
  6. Jiang SX, Wang XS, Geng CH, Wang GY. Altering trend of clinical characteristics of colorectal cancer: a report of 3,607 cases. Ai Zheng 2009; 28(1): 54-6.
  7. Paul-Samojedny M, Kokocinska D, Samojedny A, Mazurek U, Partyka R, Lorenz Z, et al. Expression of cell survival/death genes: Bcl-2 and Bax at the rate of colon cancer prognosis. Biochim Biophys Acta 2005; 1741(1-2): 25-9.
  8. O'Connell JB, Maggard MA, Ko CY. Colon cancer survival rates with the new American Joint Committee on Cancer sixth edition staging. J Natl Cancer Inst 2004; 96(19): 1420-5.
  9. Galandiuk S, Wieand HS, Moertel CG, Cha SS, Fitzgibbons RJ, Jr., Pemberton JH, et al. Patterns of recurrence after curative resection of carcinoma of the colon and rectum. Surg Gynecol Obstet 1992; 174(1): 27-32.
  10. Arber N, Hibshoosh H, Moss SF, Sutter T, Zhang Y, Begg M, et al. Increased expression of cyclin D1 is an early event in multistage colorectal carcinogenesis. Gastroenterology 1996; 110(3): 669-74.
  11. Kim HJ, Yu MH, Kim H, Byun J, Lee C. Noninvasive molecular biomarkers for the detection of colorectal cancer. BMB Rep 2008; 41(10): 685-92.
  12. Ren J, Kan A, Leong SH, Ooi LL, Jeang KT, Chong SS, et al. FAT10 plays a role in the regulation of chromosomal stability. J Biol Chem 2006; 281(16): 11413-21.
  13. Raasi S, Schmidtke G, Groettrup M. The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis. J Biol Chem 2001; 276(38): 35334-43.
  14. Canaan A, Yu X, Booth CJ, Lian J, Lazar I, Gamfi SL, et al. FAT10/diubiquitin-like protein-deficient mice exhibit minimal phenotypic differences. Mol Cell Biol 2006; 26(13): 5180-9.
  15. Duffy MJ, van DA, Haglund C, Hansson L, Holinski-Feder E, Klapdor R, et al. Tumour markers in colorectal cancer: European Group on Tumour Markers (EGTM) guidelines for clinical use. Eur J Cancer 2007; 43(9): 1348-60.
  16. Lee CG, Ren J, Cheong IS, Ban KH, Ooi LL, Yong TS, et al. Expression of the FAT10 gene is highly upregulated in hepatocellular carcinoma and other gastrointestinal and gynecological cancers. Oncogene 2003; 22(17): 2592-603.
  17. Lukasiak S, Schiller C, Oehlschlaeger P, Schmidtke G, Krause P, Legler DF, et al. Proinflammatory cytokines cause FAT10 upregulation in cancers of liver and colon. Oncogene 2008; 27(46): 6068-74.
  18. Ji F, Jin X, Jiao CH, Xu QW, Wang ZW, Chen YL. FAT10 level in human gastric cancer and its relation with mutant p53 level, lymph node metastasis and TNM staging. World J Gastroenterol 2009; 15(18): 2228-33.
  19. Lim CB, Zhang D, Lee CG. FAT10, a gene up-regulated in various cancers, is cell-cycle regulated. Cell Div 2006; 1: 20.
  20. Yan DW, Li DW, Yang YX, Xia J, Wang XL, Zhou CZ, et al. Ubiquitin D is correlated with colon cancer progression and predicts recurrence for stage II-III disease after curative surgery. Br J Cancer 2010; 103(7): 961-9.
  21. Ren J, Wang Y, Gao Y, Mehta SB, Lee CG. FAT10 mediates the effect of TNF-alpha in inducing chromosomal instability. J Cell Sci 2011; 124(Pt 21): 3665-75.
  22. Qing X, French BA, Oliva J, French SW. Increased expression of FAT10 in colon benign, premalignant and malignant epithelial neoplasms. Exp Mol Pathol 2011; 90(1): 51-4.
  23. Fan J, Yang X, Wang W, Wood WH, III, Becker KG, Gorospe M. Global analysis of stress-regulated mRNA turnover by using cDNA arrays. Proc Natl Acad Sci U S A 2002; 99(16): 10611-6.
  24. Oliva J, Bardag-Gorce F, French BA, Li J, McPhaul L, Amidi F, et al. Fat10 is an epigenetic marker for liver preneoplasia in a drug-primed mouse model of tumorigenesis. Exp Mol Pathol 2008; 84(2): 102-12.
  25. Grady WM, Carethers JM. Genomic and epigenetic instability in colorectal cancer pathogenesis. Gastroenterology 2008; 135(4): 1079-99.
  26. Zhang DW, Jeang KT, Lee CG. p53 negatively regulates the expression of FAT10, a gene upregulated in various cancers. Oncogene 2006; 25(16): 2318-27.
  27. Johnson PM, Porter GA, Ricciardi R, Baxter NN. Increasing negative lymph node count is independently associated with improved long-term survival in stage IIIB and IIIC colon cancer. J Clin Oncol 2006; 24(22): 3570-5.
  28. Wang J, Hassett JM, Dayton MT, Kulaylat MN. The prognostic superiority of log odds of positive lymph nodes in stage III colon cancer. J Gastrointest Surg 2008; 12(10): 1790-6.
Journal of Isfahan Medical School
Volume 32, Issue 291 - Serial Number 291
May and June 2014
Pages 972-981

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History

  • Receive Date: 24 March 2014
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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