A Comparative Study of the Efficiency of the New Loop-Mediated Isothermal Amplification Assay and Routine Procedures for Diagnosis of Hepatitis C Virus

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran

2 Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran

3 Assistant Professor, Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

4 Department of Microbiology AND Young Researcher's Club, Islamic Azad University, Jahrom Branch, Jahrom, Iran

Abstract

Background: Hepatitis C virus (HCV) is a major cause of chronic liver disease and a public health problem. Therefore, rapid detection and management is important. This study aimed to compare the efficiency of enzyme-linked immunosorbent assay (ELISA), nested polymerase chain reaction (nested PCR), and loop mediated isothermal amplification assay (LAMP) for detection of HCV.Methods: This experimental study conducted on 50 serum samples suspected to HCV in Chaharmahal-va-Bakhtiari province, Iran. HCV Ab detected by ELISA and genomic RNA evaluated by nested PCR and LAMP techniques. Then, efficiency of ELISA and LAMP was calculated in comparison with nested PCR.Findings: Out of total samples, 45 (90%) samples were positive for HCV by nested PCR and LAMP. All samples (100%) were positive by ELISA, but 10% (5 samples) was false positive. In addition, in comparison with nested PCR, efficiency of LAMP and ELISA were 100% and 91%, respectively.Conclusion: Our results demonstrate that based on high efficiency, speed and cost effectiveness of LAMP assay, it could be used as a fast alternative method for diagnosis of HCV.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 261 - Serial Number 261
September and October 2014
Pages 1876-1884

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History

  • Receive Date: 01 July 2013
  • Revise Date:
  • Accept Date: 06 April 2022

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