The Cytotoxic and Pro-Apoptotic Effects of Various Extracts of Allium Giganteum on MCF-7 and Hela Cell Lines

Document Type : Original Article (s)

Authors

1 Pharm D, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Here, the cytotoxicity of various Allium giganteum extracts was evaluated on MCF-7 and HeLa cell lines, to introduce novel anti-cancer agents from natural resources,Methods: Flowers of the plant were subjected to extraction in a four steps method, and the extracts were evaluated for their cytotoxicity on MCF-7 and HeLa cell lines. MTT assay was performed 48 hours. Following treatment of the cells with 10, 20, and 50 µg/ml of different extracts. The mechanism of the induced cell death was assessed by flow cytometry following Annexin/PI staining of the treated cells.Findings: The MTT results showed significant toxicity of dichloromethane, chloroform-methanol (9:1), and butanolic extracts at 50 µg/ml on MCF-7, and at 20 and 50 µg/ml on HeLa cells compared to control (P < 0.05). No significant cytotoxicity and apoptosis induction was observed following treatment with aquatic extract. The flow cytometric analysis results indicated apoptosis induction by all the three extracts on MCF-7 cells.Conclusion: The dichloromethane, chloroform-methanol (9:1), and butanolic extracts of Allium giganteum showed cytotoxic effects on the cancer cells, and up to 26% apoptosis of MCF-7 cells. Due to the desirable results of this study, extraction and identification of constituents of the extracts, especially steroidal saponin constituents, could be valuable.

Keywords

References

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History

  • Receive Date: 05 May 2021
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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