Improvement of Spermatogenesis and Fertility in Mice under Ionizing Radiation Using Chlorogenic Acid

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

2 Associate Professor, Department of Medical Physics, School of Medicine, Urmia Medical Sciences University, Urmia, Iran

3 Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IR Iran.

4 Assistant Professor, Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Abstract

Background: This study was performed to evaluate the effect of chlorogenic acid (CGA) on spermatogenesis in ionizing irradiated (IR) mice.
Methods: In this experimental study, 45 mice were divided into 3 equal groups including group 1 (normal saline), group 2 (IR + normal saline), and group 3 (IR + 5, 10, 20, 40, and 80 mg/kg CGA). After 35 days, the histomorphometry of testicular tissue, cells count in different developmental stages, viability of sperms and finally, fertilization capacity was evaluated. Data were analyzed by ANOVA one-way analysis of variance and post hoc Turkey’s tests.
Findings: The results showed that testicular weight, germinal layer thickness and diameter of seminiferous tubules in the IR group were significantly different from the sham and IR + CGA (40 mg/kg) groups. In addition, in the IR group, the number of spermatogonia cells, primary spermatocytes, spermatid and Leydig cells were significantly decreased compared to the sham group. These changes were significantly ameliorated in the IR + CGA (40 mg/kg) group and histological studies did not show any tissue damage. At the same time, the number of germ cells, the thickness of the epithelium of the seminiferous tubules and the diameter of the seminiferous tubules didn’t showe significantly difference with the sham group.
Conclusion: The present study shows that CGA (40 mg/kg) can be beneficial against the harmful effects of X-ray exposure by improving histopathological changes in the testis.

Keywords

References

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Journal of Isfahan Medical School
Volume 40, Issue 697
1st Week,, February
January and February 2023
Pages 981-990

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