A Comparison between Antioxidant Effects of Korean Red Ginseng Extract (KRGE)-Loaded Poly(Lactic-co-Glycolic Acid) (PLGA) Nanoparticles and Korean Red Ginseng Extract (KRGE) in the Treatment of Testosterone-Induced Polycystic Ovarian Syndrome

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Biology, School of Basic Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

2 Assistant Professor, Department of Biology, School of Basic Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

3 Associate Professor, Department of Biology, School of Basic Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Abstract

Background: This study aimed to evaluate and compare the effects of Korean red ginseng extract (KRGE) and KRGE-loaded poly(lactic-co-glycolic acid) nanoparticles (KRGE-loaded PLGA NPs) on testosterone-induced polycystic ovarian syndrome (PCOS) mice.Methods: Female Naval Medical Research Institute (NMRI) post-pubertal mice were divided into control (n = 10), sham (n = 10), and PCOS (n = 60) groups. PCOS was induced in sham and PCOS groups by subcutaneous injection of testosterone enanthate 1 mg/100mg/day in the back of the mice neck for 4 weeks; then, the PCOS group was intraperitoneally treated with different concentrations of KRGE and KRGE-loaded PLGA NPs for the consecutive 15 days. The ovarian tissues were surgically removed for determinations of malondialdehyde (MDA), reactive oxygen species, glutathione (GSH), total antioxidant capacity (TAC), glutathione S-transferase (GST), and qualitative real-time polymerase chain reaction (qRT-PCR) analysis to detect glutathione reductase (GR) and nuclear factor erythroid 2 (NRF-2) genes.Findings: Both KRGE and KRGE-loaded PLGA NPs were beneficial to decrease MDA, and oxidative agents as well as increase the level of GSH, TAC, and GST in PCOS mice. According to qRT-PCR analysis, the expressions of NRF-2 and GR genes were elevated in the treated mice. However, statistical analysis revealed that KRGE-loaded PLGA NPs were more effective than KRGE.Conclusion: Our results are consistent with the beneficial effect of KRGE on the treatment of PCOS. Nonetheless, KRGE coated with PLGA NPs were suggested more effective according to our findings.

Keywords

References

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Journal of Isfahan Medical School
Volume 39, Issue 650 - Serial Number 650
November and December 2022
Pages 875-882

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History

  • Receive Date: 20 August 2021
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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