Evaluation of Various Water Resources in Isfahan, Iran, for the Presence of Helicobacter Pylori Using Fluorescent Nested Polymerase Chain Reaction

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Microbiology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Helicobacter pylori infection is one of the most common infectious agents worldwide. However, origin and the mode of transmission of this bacterium have not been clearly explained. One of the most probable routs of Helicobacter pylori transmission is through water resources. The aim of this study was to evaluate the presence of Helicobacter pylori in tap waters, well waters and Zayandeh Rood River collected from various locations in Isfahan, Iran.Methods: Totally, 100 liters of water were collected from Zayandeh Rood River, wells and drinking water pipeline network in the north, west, south and west locations of Isfahan city. Collected waters first subjected to filteration through 0.25 µm, then, filters were washed with phosphate buffered saline (PBS) and the washed out PBS used for DNA extraction. For DNA extraction, QiAamp DNA Mini Kit was used, fluorescent nested polymerase chain reaction was used for detection of Helicobacter pylori genome using UreC, 16srRNA and adhesion gene specific primers.Findings: Our results showed Helicobacter pylori infection in Zayandeh Rood river, well waters and drinking water from eastern region of Isfahan.Conclusion: Using primers srRNA16, UreC, fluorescent nested polymerase chain reaction method and fluorochromes 5CY, very little number of bacteria were identified.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 298 - Serial Number 298
July and August 2014
Pages 1347-1353

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History

  • Receive Date: 04 August 2014
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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