Histhopathological Investigation of the Intraperitoneal Injection of Starch-Graft-Polyaniline/Magnesium Oxide (Chitosan/MgO) Nanocomposites on Liver and Kidney of Rats

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Basic Science, School of Veterinary Medicine, University of Zabol, Zabol, Iran

2 Associate Professor, Department of Chemistry, School of Basic Science, University of Zabol, Zabol, Iran

Abstract

Background: At the present study, we assessed the effects of intraperitoneal injections of nanocomposite of Starch-Graft-Polyaniline/Magnesium Oxide (Chitosan/MgO) on liver and kidney of male rats.Methods: 28 male rats were randomly allocated into four equal groups. The control group received 0.5 ml normal saline daily via intraperitoneal injection, and the treatment groups received daily intraperitoneal injections of Chitosan/MgO nanocomposites (1, 2, and 4 mg/ml) for four weeks. At the end of the experiment, the serum samples were taken to determine serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as serum liver markers, and serum blood urea nitrogen (BUN) and creatine as serum kidney biomarkers. After euthanasia, liver and kidney samples were taken to assess histological changes.Findings: The group receiving 4 mg/ml of Chitosan/MgO nanocomposite had a significantly higher liver enzyme levels compared to the control group (P < 0.05); however, serum BUN and creatine levels did not change compared with the control group (P > 0.05). In kidney histological analysis, mild proximal swelling was observed in treatment groups. Liver sections of the group receiving 4 mg/ml showed mild fatty changes.Conclusion: It seems that Chitosan/MgO nanocomposite had hepatotoxic and nephrotoxic effects; so, their applications in food industry and medicine should be limited.

Keywords

References

  1. Cai L, Chen J, Liu Z, Wang H, Yang H, Ding W. Magnesium oxide nanoparticles: effective agricultural antibacterial agent against Ralstonia solanacearum. Front Microbiol 2018; 9: 790.
  2. Falcaro P, Ricco R, Yazdi A, Imaz I, Furukawa S, Maspoch D, et al. Application of metal and metal oxide nanoparticles@MOFs. Coord Chem Rev 2016; 307: 237-54.
  3. Akram mW, Fakhar-e-Alam M, Butt AR, Munir T, Ali A, Alimgeer KS, et al. Magnesium oxide in nanodimension: model for MRI and multimodal therapy. J Nanomater 2018; 2018. 4210920.
  4. de Silva RT, Mantilaka MMMG, Ratnayake SP, Amaratunga GAJ, de Silva KMN. Nano-MgO reinforced chitosan nanocomposites for high performance packaging applications with improved mechanical, thermal and barrier properties. Carbohyd Polym 2017; 157: 739-47.
  5. Mangalampalli B, Dumala N, Perumalla VR, Grover P. Genotoxicity, biochemical, and biodistribution studies of magnesium oxide nano and microparticles in albino wistar rats after 28-day repeated oral exposure. Environ Toxicol 2018; 33(4): 396-410.
  6. Nogi M, Yano H. Transparent nanocomposites based on cellulose produced by bacteria offer potential innovation in the electronics device industry. Adv Mater 2008; 20(10): 1849-52.
  7. Canli EG, Ila HB, Canli M. Response of the antioxidant enzymes of rats following oral administration of metal-oxide nanoparticles (Al2O3, CuO, TiO2). Environ Sci Pollut Res Int 2019; 26(1): 938-45.
  8. Mahmoud A, Ezgi O, Merve A, Ozhan G. In vitro toxicological assessment of magnesium oxide nanoparticle exposure in several mammalian cell types. Int J Toxicol 2016; 35(4): 429-37.
  9. Kiranmai G, Reddy AR. Antioxidant status in MgO nanoparticle-exposed rats. Toxicol Ind Health 2013; 29(10): 897-903.
  10. Jahangiri L, Kesmati M, Najafzadeh H. Evaluation of analgesic and anti-inflammatory effect of nanoparticles of magnesium oxide in mice with and without ketamine. Eur Rev Med Pharmacol Sci 2013; 17(20): 2706-10.
  11. Organization for Economic Co-operation and Development(OECD). OECD 423: OECD Guideline for Testing of Chemicals- Acute Oral Toxicity - Acute Toxic Class Method [Online]. [cited 2001 Dec 17]; Available from: URL: https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf
  12. Naghsh N, Kazemi S. Effect of nano-magnesium oxide on glucose concentration and lipid profile in diabetic laboratory mice. Iran J Pharm Sci 2014; 10(3): 63-8.
  13. Gelli K, Porika M, Anreddy RNR. Assessment of pulmonary toxicity of MgO nanoparticles in rats. Environ Toxicol 2015; 30(3): 308-14.
  14. Ghadi A, Mahjoub S, Tabandeh F, Talebnia F. Synthesis and optimization of chitosan nanoparticles: Potential applications in nanomedicine and biomedical engineering. Caspian J Intern Med 2014; 5(3): 156-61.
  15. Jacobsen NR, Stoeger T, van den Brule S, Saber AT, Beyerle A, Vietti G, et al. Acute and subacute pulmonary toxicity and mortality in mice after intratracheal instillation of ZnO nanoparticles in three laboratories. Food Chem Toxicol 2015; 85: 84-95.
  16. Seyedalipour S, Fattahi R, Khanbabaee R, Abdullahpour R. The effect of MgO nanoparticles on histopathological and biomarker changes of liver injuries (ALT, ALP, and AST) in pregnant NMRI Mice. Journal of Advances in Medical and Biomedical Research 2016; 24(102): 44-56. [In Persian].
  17. Mazaheri N, Karimi A, Salavati H, Rezaei Zarchi S, Khalilian S, Rezaei Ranjbar Sardari R. Investigating the effect of intraperitoneal injection of magnesium oxide nanoparticles on the liver and kidney function of rat in vivo. J Shahid Sadoughi Univ Med Sci 2014; 22(4): 1430-8. [In Persian].
  18. Khanooki TA, Fazilati M. The toxicity effect of magnesium oxide nanoparticles. Adv Environ Biol 2014; 8(10): 969-73.
  19. Shaikh SM, K Shyama SK, Desai PV. Absorption, LD50 and effects of CoO, MgO and PbONanoparticles on mice "Mus musculus". IOSR J Environ Sci Toxicol Food Technol 2015; 9(2): 32-8.
  20. Rahdar A, Aliahmad M, Hajinezhad MR, Samani M. Xanthan gum-stabilized nano-ceria: Green chemistry based synthesis, characterization, study of biochemical alterations induced by intraperitoneal doses of nanoparticles in rat. J Mol Struct 2018; 1173: 166-72.
Journal of Isfahan Medical School
Volume 37, Issue 517 - Serial Number 517
January and February 2019
Pages 138-144

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History

  • Receive Date: 26 December 2018
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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