Evaluation of Wound Healing and Antimicrobial Properties of Hydrogel Dressings of Starch Containing Ethanolic Extract of Propolis in the Rat

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Biomaterials, Tissue Engineering and Nanotechnology, Student Research Committee, School of Advanced Medical Technologies, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Medical Technologies AND Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Polymer Science, Iran Polymer and Petrochemical Institute, Tehran, Iran

4 MSc Student, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 General Practitioner, Cancer Prevention Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: In this research, we intended to fabricate hydrogel wound dressing by solvent-casting method with combination of starch and propolis, which is suitable for wound due to their suitable properties. Current wound dressings do not have capability of protecting wound from injuries and microorganism contaminations ideally.Methods: For preparation of film, a certain amount of corn starch was mixed with deionized water and followed by the addition of glycerol in a specific ratio (4/10 dried weight of starch). The ethanolic extract of propolis was added to the aforementioned solution in ratios of 0.25, 0.5, and 1 percent. The obtained mixture was casted on polytetrafluoroethylene (PTFE) mold and their structure and features were characterized using mechanical properties, scanning electron microscope (SEM), and antimicrobial activity.Findings: The antimicrobial properties of the wound dressing showed significant improvement by increasing of propolis concentration. There was a significant difference about the rate of wound healing in group of hydrogel dressing of starch and hydroalcoholic extract of propolis compared to other group (P < 0.05).Conclusion: According to our findings, nanosilver dressing can be effective in controlling superficial wound infection.

Keywords

References

  1. Torres FG, Commeaux S, Troncoso OP. Starch-based biomaterials for wound-dressing applications. Starch 2013; 65(7-8): 543-51.
  2. Ahmed EM. Hydrogel: Preparation, characterization, and applications: A review. J Adv Res 2015; 6(2): 105-21.
  3. Mogosanu GD, Grumezescu AM. Natural and synthetic polymers for wounds and burns dressing. Int J Pharm 2014; 463(2): 127-36.
  4. Merei JM. Pediatric clean surgical wounds: Is dressing necessary? J Pediatr Surg 2004; 39(12): 1871-3.
  5. Cascone MG, Barbani N, Cristallini C, Giusti P, Ciardelli G, Lazzeri L. Bioartificial polymeric materials based on polysaccharides. J Biomater Sci Polym Ed 2001; 12(3): 267-81.
  6. Abbas KA, Khalil SK, Hussin ASM. Modified starches and their usages in selected food products: A review study. J Agric Sci 2010; 2(2): 90-100.
  7. Pal K, Banthia AK, Majumdar DK. Starch based hydrogel with potential biomedical application as artificial skin. Afr J Biomed Res 2006; 9(1): 23-9.
  8. Pal K, Banthia AK, Majumdar DK. Preparation of transparent starch based hydrogel membrane with potential application as wound dressing. Trends Biomater Artif Organs 2006; 20(1): 59-67.
  9. Filipic B, Gradisnik L, Ruzic-Sabljic E, Trtnik B, Pereyra A, Jaklic D, et al. Water soluble propolis and royal jelly enhance the antimicrobial activity of honeys and promote the growth of human macrophage cell line. J Agric Sci Technol 2016; B6: 35-47.
  10. Huang S, Zhang CP, Wang K, Li GQ, Hu FL. Recent advances in the chemical composition of propolis. Molecules 2014; 19(12): 19610-32.
  11. Pastor C, Sanchez-Gonzalez L, Chafer M, Chiralt A, CheloGonzalez-Martinez A. Physical and antifungal properties of hydroxypropylmethylcellulose based films containing propolis as affected by moisture content. Carbohydr Polym 2010; 82(14): 1174-83.
  12. Kim JI, Pant HR, Sim HJ, Lee KM, Kim CS. Electrospun propolis/polyurethane composite nanofibers for biomedical applications. Mater Sci Eng C Mater Biol Appl 2014; 44: 52-7.
  13. Bitencourt CM, Favaro-Trindade CS, Sobral PJA, Carvalho A. Gelatin-based films additivated with curcuma ethanol extract: Antioxidant activity and physical properties of films. Food Hydrocoll 2014; 40: 145-52.
  14. Torlak E, Sert D. Antibacterial effectiveness of chitosan-propolis coated polypropylene films against foodborne pathogens. Int J Biol Macromol 2013; 60: 52-5.
  15. Mandal MD, Mandal S. Honey: Its medicinal property and antibacterial activity. Asian Pac J Trop Biomed 2011; 1(2): 154-60.
  16. de Almeida EB, Cordeiro Cardoso J, Karla de Lima A, de Oliveira NL, de Pontes-Filho NT, Oliveira Lima S, et al. The incorporation of Brazilian propolis into collagen-based dressing films improves dermal burn healing. J Ethnopharmacol 2013; 147(2): 419-25.
Journal of Isfahan Medical School
Volume 35, Issue 458 - Serial Number 458
November and December 2018
Pages 1744-1748

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History

  • Receive Date: 13 December 2017
  • Revise Date: 02 November 2024
  • Accept Date: 06 April 2022

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