The Histological Changes in Endocrine Part of the Pancreas of Diabetic Rats Treated with Rosa Damascena Extract

Document Type : Original Article (s)

Authors

1 Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 MSc Student, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

4 Assistant Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Diabetes is caused by a breakdown in insulin production or its malfunction in the body. So far, many studies have been done on its treatment. The aim of this study was to investigate the histological changes in endocrine part of the pancreas of diabetic rats treated with Rosa damascena extract.Methods: In this experimental study, 100 male rats were divided into 10 groups of control, untreated diabetic, gavage stress, insulin treatment, three healthy groups receiving different doses of extract, and three diabetic groups receiving different doses of extract (300, 600, and 900 mg/kg). Finally, pancreatic tissue was removed and immunohistochemically stained with hematoxylin-eosin to count pancreatic beta cells number, and to calculate islets of Langerhans. Statistical analysis was performed using ANOVA test, and P < 0.050 was considered as statistically significant.Findings: Rosa damascena extract at 900 mg/kg, significantly increased the area of the islets of Langerhans in diabetic rats compared to the diabetic control group (P = 0.021). This dose of extract also increased the percentage of beta cells in the islets of Langerhans compared to the diabetic control group (P = 0.017).Conclusion: According to the results, using rose extract can improve tissue changes in the pancreas. This extract can probably be considered as a suitable research potential in future studies.

Keywords

References

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Journal of Isfahan Medical School
Volume 38, Issue 578 - Serial Number 578
May and June 2020
Pages 383-389

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History

  • Receive Date: 27 May 2020
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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