In-Vitro Evaluation of miR-101-5P Effect on Herpes Simplex Virus Replication

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Microbiology and Immunology, School of Medicine AND Autoimmune Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran

2 MSc Student, Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

3 MSc Student, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Associate Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 PhD Candidate, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

6 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Herpes simplex virus type 1 (HSV-1) is known worldwide for its serious disease and a kind of infection that involves nervous system throughout human lifelong. HSV-1 infection is much more considerable in immunocompromised patients and due to the growing resistance to its main drug, acyclovir, alternative treatments are required. MicroRNAs (miRNAs) regulate host and viral gene expression, post-transcriptionally. One previous study has shown that mir-101-3p expression may play role in HSV-1-infected cells.Methods: In this study, synthesized mimic hsa-miR-101-5p was transfected to HSV-1-infected Hela cells to observe its effect on HSV-1 replication via microscopic observation.Findings: Hela cells transfected by hsa-miR-101-5p produced less viral progeny, and expressed less cytopathic effects.Conclusion: Considering the effect of hsa-miR-101 in suppressing HSV-1 replication without affecting cell viability, this achievement can give us new insights in treatment of HSV-1 infection.

Keywords

References

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Journal of Isfahan Medical School
Volume 36, Issue 494 - Serial Number 494
July and August 2018
Pages 1024-1029

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History

  • Receive Date: 09 September 2018
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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