Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in Vitro

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Assistant Professor, Department of Immunology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

3 Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

4 Student, Department of Laboratory Sciences, Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

5 MSc Student, Department of Parasitology, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

6 Associate Professor, Skin Diseases and Leishmaniasis Research Center AND Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Many studies have suggested the benefits of garlic oil extracts against Leishmania parasites. Recent research has proved that these derivatives (such as allicin) are cytotoxic and therapeutic effects of garlic are in fact due to an odorless compound (S-allyl-cysteine). Considering the small number of studies in this field, we evaluated the effectiveness of S-allyl-cysteine on survival, apoptosis, and proliferation of Leishmania major promastigotes in vitro.Methods: In this study Leishmania major promastigotes (MRHO/IR/75/ER) were used. The parasites were first cultured and then treated with S-allyl-cysteine at end concentrations of 2.5, 5.0, 10.0, 20.0, 40.0, and 50.0 mM. Their survival, apoptosis, and proliferation was assessed after 72 hours.Findings: None of the concentrations of S-allyl-cysteine could induce apoptosis in parasite promastigotes. Lower concentrations (5.0 and 10.0 mM) of S-allyl-cysteine had greatest effect on proliferation. On the other hand, direct observation showed that cultures comprising S-allyl-cysteine at end concentration of 20.0, 40.0, and 50.0 mM formed more rosettes.Conclusion: The antioxidant effects of S-allyl-cysteine have been previously proved. Similarly, this study showed that some concentrations of S-allyl-cysteine could affect the survival and growth of promastigotes in culture medium. Therefore, this substance seems to be a good stimulating factor for growth of parasite promastigotes in culture media.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 225 - Serial Number 225
January and February 2013
Pages 121-130

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History

  • Receive Date: 14 January 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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