Detection Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Isolates from Clinical Samples; Narrative Review

Document Type : Review Article

Authors

1 Assistant Professor, Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran

2 Professor, Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran

Abstract

Background: The emergence of Extended-Spectrum Beta-Lactamase- and Carbapenemase- Producing Enterobacteriaceae is a threat to global health. Fast and accurate detection of these strains plays a key role in controlling nosocomial infections and effective treatment. In the present study, the detection methods of carbapenemase and broad-spectrum β-lactam-producing Enterobacteriaceae have been investigated, focusing on the summary of culture-based techniques and molecular methods.
Methods: The present study is a narrative review. The articles published between 2000 and 2022 were searched in international authoritative databases namely Scopus, PubMed, Scholar, Google, Web of Science, Science direct.
Findings: To identify Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae, conventional culture methods, biochemical methods, mass spectrometry, molecular methods, next generation sequencing, microarrays, whole genome sequencing, hybridization-based system, starch iodine assay, immunochromatography, colorimetry, mass spectrometry, electrochemical measurement, and flow cytometry were used.
Conclusion: For the detection of Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight), next-generation sequencing and whole genome sequencing are among the new and selected techniques.

Keywords

References

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Journal of Isfahan Medical School
Volume 40, Issue 688
4th Week, November
November and December 2022
Pages 743-758

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