Induction of Parkinson’s Disease Model in Rat by Rotenone

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Biological Science and Biotechnology, School of Basic Sciences, University of Kurdistan, Sanandaj, Iran

2 Assistant Professor, Department of Biological Science and Biotechnology, School of Basic Sciences, University of Kurdistan, Sanandaj, Iran

3 Assistant Professor, Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran

4 Assistant Professor, Department of Physiology and Pharmacology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

Abstract

Background: Many efforts have been made to find a proper animal model for Parkinson’s disease, in order to improve our knowledge about this disorder and to find therapeutic approaches. Generally, the reproducibility of experiments in laboratory animals is accompanied by some troubles. Therefore, the validity of model is certified via certain behavioral tests. The present study aimed to find an accurate and reproducible rat model of inducing Parkinson’s disease by rotenone.Methods: Male Wistar rats weighting 400 ± 50 g (10-12 months of age) received several doses of rotenone (1, 2, or 3 mg/kg) or its vehicle subcutaneously every 48 hours. Three behavioral tests (rotarod, rearing and bar) were run in order to check the development of model.Findings: Rotenone (2 mg/kg/48h) was an efficient dose beside its low mortality. The latency time of rotarod test and movement skills in rearing test decreased significantly in rotenone (2 mg/kg/48h) treated animals (P < 0.05). In addition, the results of bar test showed the augmentation of catalepsy in rotenone group compared to vehicle-treated animals (P < 0.05). In addition, the statistical correlation between behavioral tests justified the development of movement disorders in the model.Conclusion: According to the results, it was concluded that rotenone (2 mg/kg/48h) was the best approach with reproducible capacity for induction of Parkinson’s disease model.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 296 - Serial Number 296
July and August 2014
Pages 1250-1258

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History

  • Receive Date: 19 July 2014
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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