Phenotypic Characteristics and Antibiotic Resistance Patterns of Most Common Nosocomial Pathogens in Noor Hospital, Isfahan, Iran

Document Type : Original Article(s)

Authors

1 Assistant Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran.

2 Department of Microbiology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran.

3 Medical Student, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Abstract

Background: Nosocomial infection is a serious problem in medical science that expends a lot of money and prolongation of treatment of the patient. This study was done to find the main causes of nosocomial infection in Isfahan and their antimicrobial resistance.Methods: In this study, 108 admitted patients suspected of nosocomial infections were studied. The patients were admitted in different wards of Isfahan Noor hospital. Blood, urine, ulcer or nasal sample were sent to the laboratory for diagnosis and designing antimicrobial resistance pattern. After purification and determination of bacterial type and the disk diffusion method, E. Test, antibiotic susceptibility testing was performed. Data were analyzed by SPSS software.Results: The gram-positive bacteria, particularly Staphylococcus aureus resistant to ceftriaxone, cotrimoxazole, and cefotaxime hundred percentage and have high sensitivity to vancomycin. The high resistance among gram-negative bacteria to antibiotics investigated in this project, including ceftriaxone, cotrimoxazole and cefotaxime has been seen.Conclusion: The results obtained in this project showed the high resistance of gram-positive and gram negative isolates to the commonly recommended antibiotics. Therefore, preventive interventions of nasocomial infection should be used. In addition, consumption of antibiotics should be restricted and proper administration of sensitive antibiotics affecting Staphylococcus aureus such as vancomycin is recommended. 

Keywords

References

  1. Prevention of hospital-acquired infections. A practical guide. 2nd ed. World Health Organization; 2002.
  2. Shojaei H, Shemirani Sh. Introductory concepts of hospital epidemiology and infection control. 1st ed. Salamat; 2004.
  3. Blot S. Limiting the attributable mortality of nosocomial infection and multidrug resistance in intensive care units. Clin Microbiol Infect 2008; 14(1): 5-13.
  4. Knox KL, Holmes AH. Regulation of antimicrobial prescribing practices--a strategy for controlling nosocomial antimicrobial resistance. Int J Infect Dis 2002; 6(Suppl 1):S8-13.
  5. De Oliveira AC, Kovner CT, da Silva RS. Nosocomial infection in an intensive care unit in a Brazilian university hospital. Rev Lat Am Enfermagem 2010; 18(2):233-9.
  6. Askarian M, Hosseini SR, Kheirandish P. Incidence and microorganisms causing nosocomial infections in Ghotbeddin burn center of Shiraz, Iran, 2000-2001. Journal of Kerman University of Medical Sciences 2003;10(2):65-70.
  7. Struelens MJ. The epidemiology of antimicrobial resistance in hospital acquired infections: problems and possible solutions. BMJ 1998; 317(7159):652-4.
  8. Hambraeus A, Paardekooper C, White MC. International Federation of Infection Control: the first 10 years. Am J Infect Control 1997; 25(4): 297-302.
  9. Mashoof R, Nazari M, Samarghandi M, Shams M. The effectiveness of the power of current disinfectant on isolated Staphylococcus epidermidis and Pseudomonas aeruginosa from hospital in 1385 in Hamedan. Zahedan Journal of research in Medical Sciences 2006; 8(4):9-15.
  10. Capoor MR, Nair D, Deb M, Hasan A, Aggarwal P. A simple modification of minimum inhibitory concentration determination by E.Test in the clinical laboratory. Indian J Med Microbiol 2006; 24(4):301.
  11. Sader HS, Pignatari AC. E.Test: a novel technique for antimicrobial susceptibility testing. Sao Paulo Med J 1994; 112(4):635-8.
  12. National Committee for Clinical Laboratory Standards. Performance standard for antimicrobial disk susceptibility testing. 3rd ed. Clinical and laboratory Standards Institute (CLSI); 2008.
  13. Lorian V. Antibiotics in laboratory medicine. 5th ed. Lippincott Williams & Wilkins; 2005.
  14. Aligholi M, Emaneini M, Hashemi FB, Shasavan Sh, Jebelameli F, kazemi F. Determination of antimicrobial resistance pattern of Staphylococcus aureus isolated from clinical specimens. Tehran University Medical Journal 2007; 64(9):26-32.
  15. Shakibaei MR, Gholamalibeig AH. Plasmid Resistance in 10 Strains of Klebsiella Pneumoniae Collected from Hospitals in Kerman Against Ceftizoxime and Cefotaxime. Journal of Kerman University of Medial Sciences 1998; 6(1):29-38.
  16. Mokhtarian Dalooei H, Ghahremani M, Nourzad H. A study of antibiotic resistance of Escherichia coli isolated from urinary tract infection. Ofogh-E-Danesh 2006; 12(3):5-9.
  17. Agnihotri N, Gupta V, Joshi RM. Aerobic bacterial isolates from burn wound infections and their antibiograms--a five-year study. Burns 2004; 30(3):241-3.
  18. Ghasemian R, Najafi N, Shojai A. Nasal carriage and antibiotic resistance of Staphylococcus aureus isolates of Razi hospital personel, Qaemshahr, 1382. Journal of Mazandaran University of Medical Sciences 2004; 14(44): 79-86.
  19. Mohammadzadeh Z, Mohammadzadeh Z. Burn wound infections and susceptibility to antimicrobial agents in Imam Moosa Kazem (AS) Hospital, Isfahan. The Journal of Qazvin University of Medical Sciences 1999; 9: 78-85.
  20. Ekrami A, Kalantar E. Bacterial infections in burn patients at a burn hospital in Iran. Indian J Med Res 2007; 126(6): 541-4.
  21. Shahrzad S, Ghaziani T. Comprehensive Textbook of Iranian drugs. Tehran: Teimoorzadeh; 2003.
Journal of Isfahan Medical School
Volume 28, Issue 123 - Serial Number 123
November and December 2011
Pages 1860-1870

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History

  • Receive Date: 03 April 2011
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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