Enhanced Intracellular Translocation of Gold Nanoparticles Functionalized with TAT Peptide into Cancer Cell Lines

Document Type : Original Article (s)

Authors

1 Department of Nanotechnology, School of Biological Science, Tarbiat Modares University, Tehran, Iran

2 Professor Department of Biochemistry, School of Biological Science, Tarbiat Modares University, Tehran, Iran

3 Professor, Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran

4 Assistant Professor, Department of Nanotechnology, School of Biological Science, Tarbiat Modares University, Tehran, Iran

Abstract

Background: Reduced toxicity and ease of modification make gold nanoparticles (GNPs) suitable for targeted delivery and bioimaging via conjugating cell-penetrating peptides (CPPs). This study aimed to assess the enhanced penetration of gold nanoparticles into cells via using TAT peptide as a cell-penetrating peptide.Methods: Gold nanoparticles and TAT-conjugated gold nanoparticles (TAT-C-GNP) were characterized using ultraviolet-visible spectrophotometry, dynamic light scattering, zeta potential method, and transmission electron microscopy. The cytotoxicity effects of nanoparticles on HeLa, and A431 cell lines were studied. Uptake of TAT-conjugated gold nanoparticles was tested in cells, using dark field microscopy.Findings: Gold nanoparticles and TAT-conjugated gold nanoparticles had little to no effect on cell viability. Upon exposure to gold nanoparticles, TAT-conjugated gold nanoparticles displayed higher uptake than gold nanoparticles in A431 cell line; whereas none of the nanoparticles showed penetration in HeLa cell.Conclusion: Thus, TAT-conjugated gold nanoparticles have enhanced cellular internalization and are suitable for various biomedical applications as nanoconjugates.

Keywords

References

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Journal of Isfahan Medical School
Volume 33, Issue 351 - Serial Number 351
July and August 2015
Pages 1574-1583

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History

  • Receive Date: 08 August 2015
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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