The Effect of HIIT Exercises on the Changes of Pannexin-1, P2X7, and NLRP-1 in Heart Tissue of Male Wistar Rats with Type 2 Diabetes

Document Type : Original Article (s)

Authors

1 MSc, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Assistant Professor, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Professor, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Assistant Professor, Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: The purpose of the present study is to study the effect of HIIT exercises on the changes in the levels of Pannexin-1, P2X7, and NLRP-1 proteins in the heart tissue of male Wistar rats with type 2 diabetes.
Methods: In this research, 40 8-week-old Wistar male rats were randomly divided into four groups: healthy control + healthy exercise + diabetes exercise + control diabetes (n = 10). Diabetic mice were induced by a high-fat diet and intraperitoneal injection of streptozocin. After that, the HIIT training program consisted of 6 2-minute sessions with 80% of the peak speed in the first week, which was increased by 10% every week and continued up to 12 sessions in the seventh and eighth weeks. 48 hours after the last training session, the heart tissue was isolated under sterile conditions, and the desired protein levels were measured using the western blot method. One-way statistical method and Tukey's post hoc test were used.
Findings: The results showed that diabetes caused a significant increase in the amount of Panexin-1, P2X7, and NLRP-1 proteins in the heart tissue of diabetic rats compared to the healthy group (P ˂ 0.5). It was also found that eight weeks of HIIT training caused a significant decrease in the amounts of Panexin-1, P2X7, and NLRP-1 proteins in the heart tissue of trained diabetic rats compared to the control diabetic group (P ˂ 0.5).
Conclusion: Finally, it can be stated that HIIT exercises have a beneficial role in regulating blood sugar levels and the amounts of Pannexin-1, P2X7, and NLRP-1 proteins in the heart tissue of diabetic rats and can probably be used as a Non-pharmacological strategy is promising.

Highlights

Aliakbar Alizadeh: Google Scholar, PubMed

Amirhossein Ahmadi Ramezani: Google Scholar, PubMed

Keywords

Main Subjects

References

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Journal of Isfahan Medical School
Volume 42, Issue 769
1st Week, August
July and August 2024
Pages 439-449

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History

  • Receive Date: 23 June 2024
  • Accept Date: 17 August 2024

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