The Effect of Hydro-alcoholic Extract of Lavandula Angustifolia on Purkinje Neurons Following Enforced Swimming Stress in Male Rats

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Anatomy, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzvar, Iran

2 Instructor, Department of Physiology-Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran

3 Student of Medicine, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran

Abstract

Background: There was no report about the effect of Lavandula angustifolia (lavender) on Purkinje neurons in cerebellum. The purpose of this study was to determine the effects of lavender extract on structure of Purkinje neurons during forced swimming stress.Methods: Fifty rats were randomly divided into five groups of ten. Four groups were enforced under swimming stress; the first group received gavage normal saline, the second, third, and fourth group received 200, 400, and 600 mg/kg of gavage lavender extract, respectively. The fifth group considered as intact control. The treatment period was 14 days. Rats were deeply anesthetized and sacrificed, and the cerebella dissected and fixed in formalin. Histological passage was performed for each section, and stained thereafter. The number of normal Purkinje neurons were then counted. The morphological changes in the Purkinje neurons were histologically and immunohistochemically determined. Data were statistically analyzed using Duncan and Scheffe tests.Findings: There were significant increase in mean number of normal Purkinje neurons in rats received 400 mg/kg lavender extract compared to other groups (P < 0.001). Compared to other groups, the morphological changes such as density of the nucleus in Purkinje neurons, raised cytoplasm acidophilic, and caspase-3-positive neuronal Purkinje increased in the group with swimming stress and normal saline.Conclusion: The results showed that lavender extract at 400 mg/kg dose may protect Purkinje neurons in male rats under forced swimming stress.

Keywords

References

  1. Gradus JL. Prevalence and prognosis of stress disorders: A review of the epidemiologic literature. Clin Epidemiol 2017; 9: 251-60.
  2. Aruoma OI. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutat Res 2003; 523-524: 9-20.
  3. Schneiderman N, Ironson G, Siegel SD. Stress and health: psychological, behavioral, and biological determinants. Annu Rev Clin Psychol 2005; 1: 607-28.
  4. Wolf OT, Atsak P, de Quervain DJ, Roozendaal B, Wingenfeld K. Stress and memory: A selective review on recent developments in the understanding of stress hormone effects on memory and their clinical relevance. J Neuroendocrinol 2016; 28(8): 12353.
  5. Bottaccioli AG, Bottaccioli F, Minelli A. Stress and the psyche-brain-immune network in psychiatric diseases based on psychoneuroendocrineimmunology: A concise review. Ann N Y Acad Sci 2019; 1437(1): 31-42.
  6. Michaels CC, Holtzman SG. Early postnatal stress alters place conditioning to both mu- and kappa-opioid agonists. J Pharmacol Exp Ther 2008; 325(1): 313-8.
  7. Yarahalli J, V, Baggavalli S, Reddy D, Sistla S, Malempati R. Effect of endosulfan and bisphenol A on the expression of SUMO and UBC9. Drug Chem Toxicol 2018; 1-8.
  8. Coelho VR, Gianesini J, Von Borowski R, Mazzardo-Martins L, Martins DF, Picada JN, et al. Linalool, a naturally occurring monoterpene compound, impairs memory acquisition in the object recognition task, inhibitory avoidance test and habituation to a novel environment in rats. Phytomedicine 2011; 18(10): 896-901.
  9. Lee I, Eriksson P, Fredriksson A, Buratovic S, Viberg H. Developmental neurotoxic effects of two pesticides: Behavior and neuroprotein studies on endosulfan and cypermethrin. Toxicology 2015; 335: 1-10.
  10. Rahzani K, Malekirad A, Shariatzadeh S, Birami M, Fazli D, Baghinia M. A comparsion of the effects of anethum graveolens and wheat germ oil on the blood oxidative stress in wistar rats. J Med Plants 2009; 4(32): 79-83. [In Persian].
  11. Mirzaei F, Keshtgar S, Kaviani M, Rajaeifar AR. The effect of lavender essence smelling during labor on cortisol and serotonin plasma levels and anxiety reduction in nulliparous women. J Kerman Univ Med Sci 2009; 16(3): 245-54. [In Persian].
  12. Hritcu L, Cioanca O, Hancianu M. Effects of lavender oil inhalation on improving scopolamine-induced spatial memory impairment in laboratory rats. Phytomedicine 2012; 19(6): 529-34.
  13. Greenberg MJ, Slyer JT. Effectiveness of Silexan oral lavender essential oil compared to inhaled lavender essential oil aromatherapy for sleep in adults: a systematic review. JBI Database System Rev Implement Rep 2018; 16(11): 2109-17.
  14. Timmann D, Drepper J, Frings M, Maschke M, Richter S, Gerwig M, et al. The human cerebellum contributes to motor, emotional and cognitive associative learning. A review. Cortex 2010; 46(7): 845-57.
  15. Ito M. Historical review of the significance of the cerebellum and the role of Purkinje cells in motor learning. Ann N Y Acad Sci 2002; 978: 273-88.
  16. Vakili A, Sharifat S, Akhavan MM, Bandegi AR. Effect of lavender oil (Lavandula angustifolia) on cerebral edema and its possible mechanisms in an experimental model of stroke. Brain Res 2014; 1548: 56-62.
  17. Buyukokuroglu ME, Gepdiremen A, Hacimuftuoglu A, Oktay M. The effects of aqueous extract of Lavandula angustifolia flowers in glutamate-induced neurotoxicity of cerebellar granular cell culture of rat pups. J Ethnopharmacol 2003; 84(1): 91-4.
  18. Cardia GFE, Silva-Filho SE, Silva EL, Uchida NS, Cavalcante HAO, Cassarotti LL, et al. Effect of Lavender (Lavandula angustifolia) Essential Oil on Acute Inflammatory Response. Evid Based Complement Alternat Med 2018; 2018: 1413940.
  19. Golmohammadi R, Rakhshani MH, Moslem AR, Pejhan A. Prognostic Role of PTEN Gene Expression and Length of Survival of Breast Cancer Patients in the North East of Iran. Asian Pac J Cancer Prev 2016; 17(S3): 305-9.
  20. Dashti G, Rashidi B, Reisi P, Rah-Afrouz L. Dashti GhR, Rashidi B, Reisi P, Rah-Afrouz L. The Effect of Iron and Cholesterol on Neuronal Apoptosis in Dentate Gyrus of Hippocampus in Rabbits Fed with High-Cholesterol Diet. J Isfahan Med Sch 2015; 33(349): 1459-67. [In Persian].
  21. Gown AM, Willingham MC. Improved detection of apoptotic cells in archival paraffin sections: immunohistochemistry using antibodies to cleaved caspase 3. J Histochem Cytochem 2002; 50(4): 449-54.
  22. Aboutaleb N, Jamali H, Abolhasani M, Pazoki TH. Lavender oil (Lavandula angustifolia) attenuates renal ischemia/reperfusion injury in rats through suppression of inflammation, oxidative stress and apoptosis. Biomed Pharmacother 2019; 110: 9-19.
  23. Rezvani ME, Roohbakhsh A, Mosaddegh MH, Esmailidehaj M, Khaloobagheri F, Esmaeili H. Anticonvulsant and depressant effects of aqueous extracts of Carum copticum seeds in male rats. Epilepsy Behav 2011; 22(2): 220-5.
  24. Abbasi Maleki S, Bekhradi R, Asgharpanah J, Abbasi Maleki F, Maleki Ahanghari N. Antidepressant-Like Effect of Aqueous and Hydroalcoholic Extracts of Lavandula Angustifolia Mill in Forced Swim Test and Tail Suspension Test in Male Mice. J Arak Uni Med Sci. 2013; 16 (9) :65-75. [In Persian].
  25. Haleagrahara N, Radhakrishnan A, Lee N, Kumar P. Flavonoid quercetin protects against swimming stress-induced changes in oxidative biomarkers in the hypothalamus of rats. Eur J Pharmacol 2009; 621(1-3): 46-52.
  26. Rabiei Z, Rafieian-Kopaei M, Mokhtari S, Alibabaei Z, Shahrani M. The effect of pretreatment with different doses of Lavandula officinalis ethanolic extract on memory, learning and nociception. Biomed. Aging Pathol 2014; 4(1): 71-6.
  27. Oskouie AA, Yekta RF, Tavirani MR, Kashani MS, Goshadrou F. Lavandula angustifolia effects on rat models of Alzheimer's disease through the investigation of serum metabolic features using NMR metabolomics. Avicenna J Med Biotechnol 2018; 10(2): 83-92.
  28. Field T, Field T, Cullen C, Largie S, Diego M, Schanberg S, et al. Lavender bath oil reduces stress and crying and enhances sleep in very young infants. Early Hum Dev 2008; 84(6): 399-401.
  29. Arzi A, Ahamehe M, Sarahroodi S. Effect of hydroalcoholic extract of Lavandula officinalis on nicotine-induced convulsion in mice. Pak J Biol Sci 2011; 14(11): 634-40.
  30. Abdanipour A, Nejatbakhsh R, Jafari Anarkooli I, Ghorbanli M, Nikfar A, Noriyan A. Prolifration and Anti-Apoptotic Effect of Hydroethanolic Extract of Lavandula officinalis on Rat Neural Stem Cells. J Adv Med Biomed Res. 2016; 24 (105) :43-52. [In Persian].
  31. Jacobs HIL, Hopkins DA, Mayrhofer HC, Bruner E, van Leeuwen FW, Raaijmakers W, et al. The cerebellum in Alzheimer's disease: evaluating its role in cognitive decline. Brain 2018; 141(1): 37-47.
  32. Duan X, Tashiro M, Wu D, Yambe T, Wang Q, Sasaki T, et al. Autonomic nervous function and localization of cerebral activity during lavender aromatic immersion. Technol Health Care 2007; 15(2): 69-78.
Journal of Isfahan Medical School
Volume 37, Issue 555 - Serial Number 555
November and December 2020
Pages 1338-1346

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History

  • Receive Date: 07 February 2019
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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